Western Blot (WB)
(HBO1/MYST2 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HBO1/MYST2 antibody detected the HBO1/MYST2 protein (arrow).)
Rabbit anti-Human MYST2 Polyclonal Antibody | anti-MYST2 antibody
MYST2, ID (Histone Acetyltransferase MYST2, EC 2.3.1.48, MYST Protein 2, MOZ, YBF2/SAS3, SAS2 and TIP60 Protein 2, Histone Acetyltransferase Binding to ORC1, HBO1, HBOa) (APC)
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Western Blot (WB)
(HBO1/MYST2 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HBO1/MYST2 antibody detected the HBO1/MYST2 protein (arrow).)
Western Blot (WB)
(HBO1/MYST2 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HBO1/MYST2 antibody detected the HBO1/MYST2 protein (arrow).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with HBO1/MYST2 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with HBO1/MYST2 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with HBO1/MYST2 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HBO1/MYST2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HBO1/MYST2 immunoreactivity is localized to nucleus significantly. )
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with HBO1/MYST2 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HBO1/MYST2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HBO1/MYST2 immunoreactivity is localized to nucleus significantly. )
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is part of the multimeric HBO1 complex, which possesses histone H4-specific acetyltransferase activity. This activity is required for functional replication origins and is involved in transcriptional activation of some genes. In both cases, the acetylation of histone H4 helps unfold chromatin so that the DNA can be accessed and replicated or transcribed. [provided by RefSeq, Oct 2016]
Uniprot Description
MYST2: a histone acetyltransferase that participates in DNA replication and the loading of the minichromosome maintenance (Mcm) complex onto chromatin. Is required for origin recognition complex (Orc) formation and replication licensing, and is responsible for much of the acetylation of histone H4. Interacts with MCM2 and ORC1L. Phosphorylated by Cdc2 during mitosis, creating a site that recruits Plk1, which in turn phosphorylates MYST2. Its phosphorylation by Plk1 during mitosis may be required for the formation of the prereplicative complex. Interacts with the androgen receptor (AR) in the presence of dihydrotestosterone. Specifically represses AR-mediated transcription.
Protein type: EC 2.3.1.48; Transcription, coactivator/corepressor; Acetyltransferase; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 17q21.32
Cellular Component: nucleoplasm; cytoplasm; nucleolus; histone acetyltransferase complex
Molecular Function: protein binding; histone acetyltransferase activity; zinc ion binding; transcription factor activity
Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; DNA replication
Research Articles on MYST2
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Product Notes
The MYST2 kat7 (Catalog #AAA6489232) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYST2, ID (Histone Acetyltransferase MYST2, EC 2.3.1.48, MYST Protein 2, MOZ, YBF2/SAS3, SAS2 and TIP60 Protein 2, Histone Acetyltransferase Binding to ORC1, HBO1, HBOa) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYST2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-1:50 ELISA: 1:1,000 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MYST2 kat7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYST2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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