Rabbit MYPT1 Polyclonal Antibody | anti-MYPT1 antibody

MYPT1 (Ser668) Antibody

Cat. Num. AAA9231074

• Gene Names: PPP1R12A; MBS; M130; MYPT1
• Reactivity: Human, Mouse; Predicted: Chicken, Rat
• Applications: Western Blot, ELISA
• Synonyms: MYPT1; Polyclonal Antibody; MYPT1 (Ser668) Antibody; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit; PPP1R12A; MBS; anti-MYPT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Chicken, Rat
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This MYPT1 (Ser668) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 641-674 amino acids from human MYPT1.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 1030

• Applicable Applications for anti-MYPT1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:4000
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Cellular Location: Cytoplasm. Note=Along actomyosin filaments and stress fibers
• Tissue Location: Expressed in striated muscles, specifically in type 2a fibers (at protein level).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-MYPT1 (Ser668) Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: C2C12 whole cell lysateLane 4: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 115 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-MYPT1 antibody

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

Product Categories/Family for anti-MYPT1 antibody

Primary Antibodies; Signal Transduction

References

Takahashi N.,et al.Genomics 44:150-152 (1997). Guo J.H.,et al.Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases. Xia D.,et al.Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45 (2004). Scherer S.E.,et al.Nature 440:346-351 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 219842212
• NCBI GeneID: 4659
• NCBI Accession #: NP_001137357.1
• NCBI GenBank Nucleotide #: NM_001143885.1
• UniProt Accession #: O14974; Q2NKL4; Q569H0; Q86WU3; Q8NFR6; Q9BYH0; B4DZ09; F8VWB4
• Molecular Weight: 115281 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 12A isoform a
• NCBI Official Synonym Full Names: protein phosphatase 1 regulatory subunit 12A
• NCBI Official Symbol: PPP1R12A
• NCBI Official Synonym Symbols: MBS; M130; MYPT1
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 12A
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 12A
• UniProt Gene Name: PPP1R12A
• UniProt Synonym Gene Names: MBS; MYPT1; Myosin phosphatase target subunit 1

NCBI Description

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

MYPT1: myosin phosphatase target subunit 1, a regulatory subunit of protein phosphatase 1. Also called the myosin-binding subunit of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. Four splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 12q21.2-q21.31

Cellular Component: A band; actin cytoskeleton; centrosome; contractile fiber; cytoplasm; cytosol; focal adhesion; kinetochore; nucleoplasm; Z disc

Molecular Function: enzyme inhibitor activity; phosphatase regulator activity; protein binding; protein kinase binding

Biological Process: centrosome organization and biogenesis; G2/M transition of mitotic cell cycle; mitosis; negative regulation of catalytic activity; positive regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; regulation of cell adhesion