Rabbit Myosin Light Chain 2 Polyclonal Antibody | anti-MYL2 antibody

Myosin Light Chain 2 (MLC2, Myosin Regulatory Light Chain, MRLC, RLC, LC20)

Cat. Num. AAA633638

• Gene Names: MYL2; MLC2; CMH10
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Myosin Light Chain 2; Polyclonal Antibody; Myosin Light Chain 2 (MLC2; Myosin Regulatory Light Chain; MRLC; RLC; LC20); Anti -Myosin Light Chain 2 (MLC2; anti-MYL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of mouse total Myosin Light Chain 2. Species Crossreactivity: human, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-MYL2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:500-1:1,000
• Immunogen: Synthetic non-phosphopeptide corresponding to mouse Myosin Light Chain 2 around the phosphorylation site of serine 18 (A-T-SP-N-V).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-MYL2 antibody

The muscle myosins and hexomeric proteins consisting of two heavy chains and two pairs of light chains, the latter called essential (ELC) and regulatory (RLC). The light chains stabilize the long alpha helical neck of the myosin head. Human myosin light chain-2 (MYL2) is an important protein involved in the regulation of myosin ATPase activity in smooth muscle. In cardiac muscle, the precise role of MYL2 is not well understood; however, an increase in ventricular MYL2 is observed during myocardial hypertrophy in cardiac patients with valve stenosis. Familial hypertrophic cardiomyopathy (FHC) is an autosomal dominant disease caused by mutations in all of the major sarcomeric proteins, including the ventricular myosin regulatory light-chain (RLC). The Myosin RLC mutation has been associated with a rare variant of cardiac hypertrophy defined by mid-left ventricular obstruction due to papillary muscle hypertrophy. This mutation was later found to cause ventricular and septal hypertrophy.

Product Categories/Family for anti-MYL2 antibody

Antibodies; Abs to Myosin

NCBI and Uniprot Product Information

• NCBI GI #: 94981553
• NCBI GeneID: 4633
• NCBI Accession #: NP_000423.2
• NCBI GenBank Nucleotide #: NM_000432.3
• UniProt Accession #: P10916; Q16123
• Molecular Weight: 18,789 Da
• NCBI Official Full Name: myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• NCBI Official Synonym Full Names: myosin, light chain 2, regulatory, cardiac, slow
• NCBI Official Symbol: MYL2
• NCBI Official Synonym Symbols: MLC2; CMH10
• NCBI Protein Information: myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; RLC of myosin; myosin light chain 2; regulatory light chain of myosin; cardiac ventricular myosin light chain 2; slow cardiac myosin regulatory light chain 2; myosin, light polypeptide 2, regulatory, cardiac, slow
• UniProt Protein Name: Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• Protein Family: Myosin
• UniProt Gene Name: MYL2
• UniProt Synonym Gene Names: MLC-2; MLC-2v
• UniProt Entry Name: MLRV_HUMAN

NCBI Description

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Protein type: Contractile

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: sarcomere; cytoskeleton; myofibril; myosin complex; cytosol; A band; actin cytoskeleton

Molecular Function: actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding

Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; muscle filament sliding; post-embryonic development; cardiac muscle contraction

Disease: Cardiomyopathy, Familial Hypertrophic, 10