Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Myosin IF (MYO1F) Polyclonal Antibody | anti-MYO1F antibody
Polyclonal Antibody to Myosin IF (MYO1F)
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Myosin IF (MYO1F); Polyclonal Antibody; Polyclonal Antibody to Myosin IF (MYO1F); anti-MYO1F antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MYO1F. It has been selected for its ability to recognize MYO1F in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-EHFNSWSAGF VIHHYAGKVS YDVSGFCERN RDVLFSDLIE LMQTSEQAFL RMLFPEKLDG DKKGRPSTAG SKIKKQANDL VATLMRCTPH YIRCIKPNET KRPRDWEENR VKHQVEYLGL KENIRVRRAG FAYRRQFAKF LQRYAILTPE TWPRWRGDER QGVQHLLRAV NMEPDQYQMG STKVFVKNPE SLFLLEEVRE RKFDGFARTI QKAWRRHVAV RKYEEMREEA SNILLNKKER RRNSINRNFV GDYLGLEERP ELRQFLGKRE RVDFADS
MGHHHHHHSGSEF-EHFNSWSAGF VIHHYAGKVS YDVSGFCERN RDVLFSDLIE LMQTSEQAFL RMLFPEKLDG DKKGRPSTAG SKIKKQANDL VATLMRCTPH YIRCIKPNET KRPRDWEENR VKHQVEYLGL KENIRVRRAG FAYRRQFAKF LQRYAILTPE TWPRWRGDER QGVQHLLRAV NMEPDQYQMG STKVFVKNPE SLFLLEEVRE RKFDGFARTI QKAWRRHVAV RKYEEMREEA SNILLNKKER RRNSINRNFV GDYLGLEERP ELRQFLGKRE RVDFADS
Sequence Length
1098
Applicable Applications for anti-MYO1F antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant MYO1F (Glu491~Ser767) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2071509
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
124,844 Da
NCBI Official Full Name
unconventional myosin-If isoform 1
NCBI Official Synonym Full Names
myosin IF
NCBI Official Symbol
MYO1F
NCBI Protein Information
unconventional myosin-If
UniProt Protein Name
Unconventional myosin-If
Protein Family
UniProt Gene Name
MYO1F
NCBI Description
Myosins are molecular motors that use the energy from ATP hydrolysis to generate force on actin filaments. The protein encoded by this gene is an unconventional myosin that may be involved in the intracellular movement of membrane-enclosed compartments. There is evidence to suggest that mutations in this gene can result in hearing loss. [provided by RefSeq, Jan 2017]
Uniprot Description
MYO1F: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments.
Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: unconventional myosin complex
Molecular Function: actin binding; ATP binding; calmodulin binding; motor activity
Research Articles on MYO1F
Similar Products
Product Notes
The MYO1F myo1f (Catalog #AAA2003386) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Myosin IF (MYO1F) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Myosin IF (MYO1F) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the MYO1F myo1f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-EHFNSW SAGF VIHHYAGKVS YDVSGFCERN RDVLFSDLIE LMQTSEQAFL RMLFPEKLDG DKKGRPSTAG SKIKKQANDL VATLMRCTPH YIRCIKPNET KRPRDWEENR VKHQVEYLGL KENIRVRRAG FAYRRQFAKF LQRYAILTPE TWPRWRGDER QGVQHLLRAV NMEPDQYQMG STKVFVKNPE SLFLLEEVRE RKFDGFARTI QKAWRRHVAV RKYEEMREEA SNILLNKKER RRNSINRNFV GDYLGLEERP ELRQFLGKRE RVDFADS. It is sometimes possible for the material contained within the vial of "Myosin IF (MYO1F), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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