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Western Blot (WB) (Western Blot: Sample: Rat Skeletal muscle lysate; Primary Ab: 1ug/ml Rabbit Anti-Human MYO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Human Myoglobin (MYO) Polyclonal Antibody | anti-MYO antibody

Polyclonal Antibody to Myoglobin (MYO)

Gene Names
MB; PVALB; myoglobgin
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity
Synonyms
Myoglobin (MYO); Polyclonal Antibody; Polyclonal Antibody to Myoglobin (MYO); anti-MYO antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MYO. It has been selected for its ability to recognize MYO in immunohistochemical staining andwestern blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,50% glycerol.
Concentration
0.4mg/ml (varies by lot)
Sequence Length
154
Applicable Applications for anti-MYO antibody
WB; IHC; ICC; IP.
Application Notes
Western blotting: 0.5-2 Ug/mL;1:200-800
Immunohistochemistry: 5-20 Ug/mL;1:20-80
Immunocytochemistry: 5-20 Ug/mL;1:20-80
Immunogen
Recombinant MYO (Met1~Gly154) expressed in E.coli
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Human, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2042879
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Rat Skeletal muscle lysate; Primary Ab: 1ug/ml Rabbit Anti-Human MYO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Rat Skeletal muscle lysate; Primary Ab: 1ug/ml Rabbit Anti-Human MYO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,184 Da
NCBI Official Full Name
myoglobin
NCBI Official Synonym Full Names
myoglobin
NCBI Official Symbol
MB
NCBI Official Synonym Symbols
PVALB; myoglobgin
NCBI Protein Information
myoglobin
UniProt Protein Name
Myoglobin
UniProt Gene Name
MB

NCBI Description

This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: cytosol

Molecular Function: heme binding; metal ion binding; oxygen binding; oxygen carrier activity

Biological Process: brown fat cell differentiation; enucleate erythrocyte differentiation; heart development; oxygen transport; response to hormone; response to hydrogen peroxide; response to hypoxia; slow-twitch skeletal muscle fiber contraction

Research Articles on MYO

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Product Notes

The MYO mb (Catalog #AAA2002968) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Myoglobin (MYO) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Myoglobin (MYO) can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2 Ug/mL;1:200-800 Immunohistochemistry: 5-20 Ug/mL;1:20-80 Immunocytochemistry: 5-20 Ug/mL;1:20-80 . Researchers should empirically determine the suitability of the MYO mb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myoglobin (MYO), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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