Western Blot (WB)
(Anti-Myoglobin antibody, MBS176166, Western blottingLane 1: Human Placenta Tissue LysateLane 2: Human Placenta Tissue Lysate )
Rabbit anti-Human Myoglobin Polyclonal Antibody | anti-MB antibody
Anti-Myoglobin antibody
Gene Names
MB; PVALB; myoglobgin
Reactivity
Human
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
Myoglobin; Polyclonal Antibody; Anti-Myoglobin antibody; myoglobin; MB antibody; MGC13548 antibody; MYG_HUMAN antibody; Myoglobin antibody; PVALB antibody; anti-MB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
154
Applicable Applications for anti-MB antibody
Western Blot (WB)
Application Notes
Western Blot
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Myoglobin (138-154aa LFRKDMASNYKELGFQG).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-Myoglobin antibody, MBS176166, Western blottingLane 1: Human Placenta Tissue LysateLane 2: Human Placenta Tissue Lysate )
Western Blot (WB)
(Anti-Myoglobin antibody, MBS176166, Western blottingLane 1: Human Placenta Tissue LysateLane 2: Human Placenta Tissue Lysate )
Related Product Information for anti-MB antibody
Description: Rabbit IgG polyclonal antibody for Myoglobin(MB) detection. Tested with WB in Human.
Background: Myoglobin(MB) also known as PVALB, is a single-chain globular protein of 153 or 154 amino acids, containing a heme (iron-containing porphyrin)prosthetic group in the center around which the remaining apoprotein folds. Two structural variants of myoglobin were described by Boyer et al.(1963). Boulton et al.(1969) studied postmortem muscle from 2,500 persons. Twomyoglobin variants were found, and in one of these substitution of lysine for glutamic acid at residue 53 was demonstrated. Myoglobin was the first protein to have its three-dimensional structure revealed. Jeffreys et al.(1984) used DNA probes isolated from the cloned myoglobin gene to map the gene in human-rodent somatic cell hybrids. The myoglobinlocus mapped to chromosome 22q11-q13. Myoglobin is released from damaged muscle tissue(rhabdomyolysis), which has very high concentrations of myoglobin. The released myoglobin is filtered by the kidneys but is toxic to the renal tubular epithelium and so may cause acute renal failure.
Background: Myoglobin(MB) also known as PVALB, is a single-chain globular protein of 153 or 154 amino acids, containing a heme (iron-containing porphyrin)prosthetic group in the center around which the remaining apoprotein folds. Two structural variants of myoglobin were described by Boyer et al.(1963). Boulton et al.(1969) studied postmortem muscle from 2,500 persons. Twomyoglobin variants were found, and in one of these substitution of lysine for glutamic acid at residue 53 was demonstrated. Myoglobin was the first protein to have its three-dimensional structure revealed. Jeffreys et al.(1984) used DNA probes isolated from the cloned myoglobin gene to map the gene in human-rodent somatic cell hybrids. The myoglobinlocus mapped to chromosome 22q11-q13. Myoglobin is released from damaged muscle tissue(rhabdomyolysis), which has very high concentrations of myoglobin. The released myoglobin is filtered by the kidneys but is toxic to the renal tubular epithelium and so may cause acute renal failure.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
17,184 Da
NCBI Official Full Name
Myoglobin
NCBI Official Synonym Full Names
myoglobin
NCBI Official Symbol
MB
NCBI Official Synonym Symbols
PVALB; myoglobgin
NCBI Protein Information
myoglobin
UniProt Protein Name
Myoglobin
Protein Family
UniProt Gene Name
MB
UniProt Entry Name
MYG_HUMAN
NCBI Description
This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family.
Protein type: Carrier
Chromosomal Location of Human Ortholog: 22q13.1
Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity
Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; response to hormone stimulus; brown fat cell differentiation; response to hypoxia; enucleate erythrocyte differentiation
Research Articles on MB
Similar Products
Product Notes
The MB mb (Catalog #AAA176166) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Myoglobin antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Myoglobin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml Tested Species: Hu Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the MB mb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myoglobin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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