MyoD Polyclonal Antibody | anti-MyoD antibody

MyoD (Phospho-Ser200)

Cat. Num. AAA191088

• Gene Names: MYOD1; PUM; MYF3; MYOD; bHLHc1
• Applications: Immunoblot, Western Blot
• Synonyms: MyoD; Polyclonal Antibody; MyoD (Phospho-Ser200); anti-MyoD antibody

• Clonality: Polyclonal
• Specificity: This antibody detects endogenous human, mouse, and rat MyoD only when phosphorylated at serine 200.
• Form/Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
• Sequence Length: 320

• Applicable Applications for anti-MyoD antibody: Immunoblotting (IB)
• Application Notes: Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~40kDa is detected.; Dilute in PBS or medium that is identical to that used in the assay system.
• Antigen: Peptide sequence that includes phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from human MyoD and conjugated to KLH.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Can be stored at 4 degree C for short-term.

Testing Data

Related Product Information for anti-MyoD antibody

MyoD encodes a nuclear protein that is a member of the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest which is required for myogenic initiation. MyoD is also involved in muscle regeneration. It activates its own transcription which may stabilize the commitment to myogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 23111009
• NCBI GeneID: 4654
• NCBI Accession #: NP_002469.2
• NCBI GenBank Nucleotide #: NM_002478.4
• UniProt Accession #: P15172; O75321
• Molecular Weight: 34,501 Da
• NCBI Official Full Name: myoblast determination protein 1
• NCBI Official Synonym Full Names: myogenic differentiation 1
• NCBI Official Symbol: MYOD1
• NCBI Official Synonym Symbols: PUM; MYF3; MYOD; bHLHc1
• NCBI Protein Information: myoblast determination protein 1
• UniProt Protein Name: Myoblast determination protein 1
• Protein Family: Myosin
• UniProt Gene Name: MYOD1
• UniProt Synonym Gene Names: BHLHC1; MYF3; MYOD; bHLHc1; Myf-3

NCBI Description

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.

Protein type: DNA-binding; RNA splicing; Transcription factor

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; chromatin DNA binding; nuclear hormone receptor binding; protein binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding

Biological Process: muscle cell fate commitment; muscle development; myotube cell development; positive regulation of muscle cell differentiation; positive regulation of myoblast differentiation; positive regulation of skeletal muscle fiber development; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of RNA splicing; regulation of transcription from RNA polymerase II promoter; skeletal muscle development