Rabbit MYOCD Polyclonal Antibody | anti-MYOCD antibody

MYOCD Antibody

Cat. Num. AAA9629021

• Gene Names: MYOCD; MYCD
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MYOCD; Polyclonal Antibody; MYOCD Antibody; MYCD; MYCD_HUMAN; Myocardin; Myocd; anti-MYOCD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: MYOCD Antibody detects endogenous levels of total MYOCD.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-MYOCD antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)(peptide)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500; ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human MYOCD, corresponding to a region within the internal amino acids.
• Conjugation: Unconjugated
• Similarity: The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation (By similarity).
• Post Translational Modifications: Phosphorylation regulates negatively the intrinsic myocardin transcriptional activity.
• Subunit Structure: Homodimer. Interacts with SRF, its association does not depend on specific DNA sequences for ternary complex formation (By similarity). Interacts with MLLT7/FOXO4. Interacts (via C-terminal) with EP300 (via the CREB-binding domain). Interacts with HDAC4 and HDAC5 (By similarity). Interacts with MEF2C (By similarity).
• Tissue Specificity: Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.
• Function: Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis) (By similarity).
• Preparation and Storage: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Related Product Information for anti-MYOCD antibody

Transcriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.

Product Categories/Family for anti-MYOCD antibody

Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 23957692
• NCBI GeneID: 93649
• NCBI Accession #: NP_705832
• NCBI GenBank Nucleotide #: NM_153604.3
• UniProt Accession #: Q8IZQ8; Q5UBU5; Q8N7Q1
• Molecular Weight: 106,769 Da
• NCBI Official Full Name: myocardin isoform 2
• NCBI Official Synonym Full Names: myocardin
• NCBI Official Symbol: MYOCD
• NCBI Official Synonym Symbols: MYCD
• NCBI Protein Information: myocardin
• UniProt Protein Name: Myocardin
• Protein Family: Myocardin
• UniProt Gene Name: MYOCD
• UniProt Synonym Gene Names: MYCD

NCBI Description

This gene encodes a nuclear protein, which is expressed in heart, aorta, and in smooth muscle cell-containing tissues. It functions as a transcriptional co-activator of serum response factor (SRF) and modulates expression of cardiac and smooth muscle-specific SRF-target genes, and thus may play a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis) ().