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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MYL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

Rabbit MYL1 Polyclonal Antibody | anti-MYL1 antibody

MYL1 Polyclonal Antibody

Gene Names
MYL1; MLC1F; MLC3F
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Synonyms
MYL1; Polyclonal Antibody; MYL1 Polyclonal Antibody; anti-MYL1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence Length
150
Applicable Applications for anti-MYL1 antibody
Western Blot (WB)
Application Notes
WB 1:500 - 1:2000
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human MYL1
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MYL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MYL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate using MYL1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate using MYL1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using MYL1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach using MYL1 antibody at dilution of 1:100 (40x lens).)
Related Product Information for anti-MYL1 antibody
Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21kDa
NCBI Official Full Name
myosin light chain 1/3, skeletal muscle isoform isoform 1f
NCBI Official Synonym Full Names
myosin, light chain 1, alkali; skeletal, fast
NCBI Official Symbol
MYL1
NCBI Official Synonym Symbols
MLC1F; MLC3F
NCBI Protein Information
myosin light chain 1/3, skeletal muscle isoform
UniProt Protein Name
Myosin light chain 1/3, skeletal muscle isoform
Protein Family
UniProt Gene Name
MYL1
UniProt Synonym Gene Names
MLC1/MLC3; MLC1F/MLC3F; Myosin light chain A1/A2
UniProt Entry Name
MYL1_HUMAN

NCBI Description

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MYL1: Regulatory light chain of myosin. Does not bind calcium. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Contractile

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: sarcomere; myofibril; muscle myosin complex; cytosol

Molecular Function: structural constituent of muscle; calcium ion binding

Biological Process: muscle contraction; cardiac muscle contraction; muscle filament sliding

Research Articles on MYL1

Similar Products

Product Notes

The MYL1 myl1 (Catalog #AAA9127915) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYL1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MYL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB 1:500 - 1:2000. Researchers should empirically determine the suitability of the MYL1 myl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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