Rabbit anti-Human, Mouse MYH6 Polyclonal Antibody | anti-MYH6 antibody

MYH6 Antibody (Center)

Cat. Num. AAA9216307

• Gene Names: MYH6; ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: MYH6; Polyclonal Antibody; MYH6 Antibody (Center); Purified Rabbit Polyclonal Antibody (Pab); Myosin-6; Myosin heavy chain 6; Myosin heavy chain; cardiac muscle alpha isoform; MyHC-alpha; MYHCA; anti-MYH6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This MYH6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 833-867 amino acids from the Central region of human MYH6.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

• Applicable Applications for anti-MYH6 antibody: Western Blot (WB), ELISA
• Application Notes: WB ~~ 1:2000
• Clone Names: RB49145
• Function: Muscle Contraction
• Cellular Location: Cytoplasm, myofibril. Note=Thick filaments of the myofibrils.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-MYH6 Antibody (Center) at 1:2000 dilutionLane 1: human skeletal muscle lysateLane 2: mouse skeletal muscle lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 224 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Product Categories/Family for anti-MYH6 antibody

Cardiovascular; Immunology; Signal Transduction

References

Matsuoka R.,et al.Am. J. Med. Genet. 41:537-547(1991). Epp T.A.,et al.Genomics 18:505-509(1993). Heilig R.,et al.Nature 421:601-607(2003). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Yamauchi-Takihara K.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:3504-3508(1989).

NCBI and Uniprot Product Information

• NCBI GI #: 156104908
• NCBI GeneID: 4624
• NCBI Accession #: NP_002462.2
• NCBI GenBank Nucleotide #: NM_002471.3
• UniProt Accession #: P13533; Q13943; Q14906; Q14907; A2RTX1; D9YZU2
• Molecular Weight: 223735
• NCBI Official Full Name: myosin-6
• NCBI Official Synonym Full Names: myosin, heavy chain 6, cardiac muscle, alpha
• NCBI Official Symbol: MYH6
• NCBI Official Synonym Symbols: ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC
• NCBI Protein Information: myosin-6
• UniProt Protein Name: Myosin-6
• Protein Family: Myosin
• UniProt Gene Name: MYH6
• UniProt Synonym Gene Names: MYHCA; MyHC-alpha
• UniProt Entry Name: MYH6_HUMAN

NCBI Description

Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located ~4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3. [provided by RefSeq, Mar 2010]

Uniprot Description

MYH6: Muscle contraction. Defects in MYH6 are the cause of atrial septal defect type 3 (ASD3). ASD3 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Defects in MYH6 are the cause of familial hypertrophic cardiomyopathy type 14 (CMH14). It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations,and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in MYH6 are the cause of cardiomyopathy dilated type 1EE (CMD1EE). It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in MYH6 are the cause of susceptibility to sick sinus syndrome type 3 (SSS3). The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors. MYH6 variations are associated with susceptibility to sick sinus syndrome (PubMed:21378987). The lifetime risk of being diagnosed with sick sinus syndrome is higher for carriers of variant p.Arg721Trp than for non-carriers (PubMed:21378987).

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: nucleoplasm; sarcomere; focal adhesion; myofibril; mitochondrion; cytoplasm; stress fiber; muscle myosin complex; cytosol; myosin complex; Z disc

Molecular Function: calmodulin binding; microfilament motor activity; protein homodimerization activity; protein heterodimerization activity; ATPase activity; actin binding; actin-dependent ATPase activity; protein kinase binding; calcium-dependent ATPase activity; ATP binding

Biological Process: adult heart development; striated muscle contraction; atrial cardiac muscle morphogenesis; regulation of heart rate; in utero embryonic development; sarcomere organization; Wnt receptor signaling pathway through beta-catenin; regulation of heart contraction; muscle filament sliding; visceral muscle development; BMP signaling pathway; ATP metabolic process; regulation of ATPase activity; myofibril assembly; muscle contraction; cardiac muscle fiber development; response to heat; regulation of blood pressure; ventricular cardiac muscle morphogenesis; regulation of the force of heart contraction; cardiac muscle contraction

Disease: Cardiomyopathy, Familial Hypertrophic, 14; Sick Sinus Syndrome 3, Susceptibility To; Cardiomyopathy, Dilated, 1ee; Atrial Septal Defect 3; Cardiomyopathy, Familial Hypertrophic, 1