Rabbit MYH6/7 Polyclonal Antibody | anti-MYH6/7 antibody

MYH6/7 Polyclonal Antibody

Cat. Num. AAA3007172

• Gene Names: MYH6; ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MYH6/7; Polyclonal Antibody; MYH6/7 Polyclonal Antibody; Myosin-6; Myosin heavy chain 6; Myosin heavy chain; cardiac muscle alpha isoform; MyHC-alpha; MYH6; MYHCA; Myosin-7; Myosin heavy chain 7; Myosin heavy chain slow isoform; MyHC-slow; cardiac muscle beta isoform; MyHC-beta; MYH7; MYHCB; anti-MYH6/7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MYH6/7 polyclonal antibody detects endogenous levels of MYH6/7 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1939

• Applicable Applications for anti-MYH6/7 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human MYH6.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MYH6/7 polyclonal antibody at 1:500 dilutionLane1:The Heart tissue lysate of Rat (40ug) Lane2:The Heart tissue lysate of Mouse (5ug))

Related Product Information for anti-MYH6/7 antibody

Myosin is a highly conserved, ubiquitously expressed protein that interacts with actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of nonphosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of myosin light chain (MLC) by myosin light chain kinase. Myosin heavy chains, which are encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle.

NCBI and Uniprot Product Information

• NCBI GI #: 156104908
• NCBI GeneID: 4624
• NCBI Accession #: NP_002462.2
• NCBI GenBank Nucleotide #: NP_002462.2
• UniProt Accession #: P13533; P12883
• Molecular Weight: ~ 260kDa
• NCBI Official Full Name: myosin-6
• NCBI Official Synonym Full Names: myosin heavy chain 6
• NCBI Official Symbol: MYH6
• NCBI Official Synonym Symbols: ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC
• NCBI Protein Information: myosin-6
• UniProt Protein Name: Myosin-6
• UniProt Gene Name: MYH6
• UniProt Synonym Gene Names: MYHCA; MyHC-alpha
• UniProt Entry Name: MYH6_HUMAN

NCBI Description

Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3. [provided by RefSeq, Feb 2017]

Uniprot Description

MYH6: Muscle contraction. Defects in MYH6 are the cause of atrial septal defect type 3 (ASD3). ASD3 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Defects in MYH6 are the cause of familial hypertrophic cardiomyopathy type 14 (CMH14). It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations,and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in MYH6 are the cause of cardiomyopathy dilated type 1EE (CMD1EE). It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in MYH6 are the cause of susceptibility to sick sinus syndrome type 3 (SSS3). The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors. MYH6 variations are associated with susceptibility to sick sinus syndrome (PubMed:21378987). The lifetime risk of being diagnosed with sick sinus syndrome is higher for carriers of variant p.Arg721Trp than for non-carriers (PubMed:21378987).

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: nucleoplasm; sarcomere; focal adhesion; myofibril; cytoplasm; stress fiber; muscle myosin complex; Z disc; myosin complex; cytosol

Molecular Function: microfilament motor activity; calmodulin binding; ATPase activity; actin-dependent ATPase activity; actin binding; protein kinase binding; ATP binding

Biological Process: adult heart development; striated muscle contraction; regulation of heart rate; metabolic process; in utero embryonic development; atrial cardiac muscle morphogenesis; Wnt receptor signaling pathway through beta-catenin; sarcomere organization; regulation of heart contraction; muscle filament sliding; visceral muscle development; BMP signaling pathway; regulation of ATPase activity; myofibril assembly; muscle contraction; cardiac muscle fiber development; regulation of blood pressure; ventricular cardiac muscle morphogenesis; regulation of the force of heart contraction

Disease: Cardiomyopathy, Familial Hypertrophic, 14; Sick Sinus Syndrome 3, Susceptibility To; Cardiomyopathy, Dilated, 1ee; Atrial Septal Defect 3; Cardiomyopathy, Familial Hypertrophic, 1