Immunohistochemistry (IHC)
(IHC of MPO-101 AP with lung tissue. 1 :200 antibody dilution in DiluObuffer.)
Rabbit Myeloperoxidase Polyclonal Antibody | anti-MPO antibody
Myeloperoxidase Antibody FITC-Conjugated N-epitope
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunocytochemistry: 1:200
Immunofluorescence: 1:200
Immunohistochemistry: 1:200
Immunoprecipitation: 1:200
Western Blot: 1:500
Immunohistochemistry (IHC)
(IHC of MPO-101 AP with lung tissue. 1 :200 antibody dilution in DiluObuffer.)
Immunohistochemistry (IHC)
(IHC of MPO-101 AP with lung tissue. 1 :200 antibody dilution in DiluObuffer.)
Western Blot (WB)
(WB of MPO-101AP with RAW cells. 1 :500 antibody dilution in DiluOButfer.)
Western Blot (WB)
(WB of MPO-101AP with RAW cells. 1 :500 antibody dilution in DiluOButfer.)
Myeloperoxidase (MPO) is a functionally important component of the normal human neutrophil azurophil granule of polymorphonuclear leukocyte’s host defense system. MPO is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. The inflammatory component of allergic reactions can be measured by eosinophil cationic protein (ECP, specific for eosinophils) and myeloperoxidase (MPO, specific for neutrophils) measurement (1). Neutrophils, especially in acute infection or the myeloid leukemias, may shed platelet-sized particles that can readily be distinguished from true platelets because they contain neutrophil myeloperoxidase, an enzyme that is not inhibited by glutaraldehyde. Traditionally Myeloperoxidase was considered as a serological marker for granulomatosis (Churg-Strauss syndrome), the main target of anti-neutrophil cytoplasm antibodies (ANCA), Low to moderate anti-Myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently it was shown that MPO participates in the initiation and progression of cardiovascular disease. It possesses potent pro-inflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is uncertain systemic vasculitis e.g. periarteritis nodosa, microscopic polyarteritis and pulmonary eosinophilic grander consideration as one of the most promising cardiac markers.
Human myeloperoxidase, a 745 amino acid protein, is produced by a gene composed of 12 exons and 11 introns. The sequence was found to contain an open reading frame, 2,235 nucleotides coding for a protein of 745 amino acids with a calculated Mr of 83,868. The heavy chain of myeloperoxidase, consisting of 467 amino acids, was located on the COOH terminus half of the protein. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 - 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2, each of which is associated with a heme-like prosthetic group. The heme environment, defined by X-ray crystallographic analyses of MPO appears to be highly conserved in four mammalian peroxidases (lactoperoxidase, thyroid, eosinophil and Myeloperoxidase). MPO when heated under non- reducing, denaturing conditions, MPO is cleaved to produced 22 and a 38kDa proteins, these two species are produced via a novel autolytic cleavage at Meth409 and Pro410 (2).
NCBI and Uniprot Product Information
NCBI Description
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]
Uniprot Description
MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.11.2.2; Oxidoreductase
Chromosomal Location of Human Ortholog: 17q23.1
Cellular Component: azurophil granule; extracellular space; lysosome; mitochondrion; nucleus; secretory granule
Molecular Function: chromatin binding; heme binding; heparin binding; metal ion binding; peroxidase activity
Biological Process: aging; defense response; defense response to fungus; hydrogen peroxide catabolic process; negative regulation of apoptosis; removal of superoxide radicals; respiratory burst during defense response; response to food; response to lipopolysaccharide; response to mechanical stimulus; response to oxidative stress; response to yeast
Disease: Alzheimer Disease; Myeloperoxidase Deficiency
Research Articles on MPO
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Product Notes
The MPO mpo (Catalog #AAA542127) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Myeloperoxidase Antibody FITC-Conjugated N-epitope reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Myeloperoxidase can be used in a range of immunoassay formats including, but not limited to, Confocal Microscopy (CM), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Confocal Microscopy: 1:200 Dot Blot: 1:10,000 ELISA: 1:10,000 Immunocytochemistry: 1:200 Immunofluorescence: 1:200 Immunohistochemistry: 1:200 Immunoprecipitation: 1:200 Western Blot: 1:500. Researchers should empirically determine the suitability of the MPO mpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myeloperoxidase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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