Rabbit anti-Human, Mouse MyD88 Polyclonal Antibody | anti-MyD88 antibody

MyD88 (Myeloid Differentiation Marker 88, Myeloid Differentiation Primary Response Gene (88), Myeloid Differentiation Primary Response Protein MyD88, MYD88D) (Azide free) (HRP)

Cat. Num. AAA6488823

• Gene Names: MYD88; MYD88D
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: MyD88; Polyclonal Antibody; MyD88 (Myeloid Differentiation Marker 88; Myeloid Differentiation Primary Response Gene (88); Myeloid Differentiation Primary Response Protein MyD88; MYD88D) (Azide free) (HRP); anti-MyD88 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MyD88. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MyD88 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human MyD88 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MyD88 Antibody (Center) in mouse lung tissue lysates (35ug/lane). MyD88 (arrow) was detected using the purified Pab.(2ug/ml))

Western Blot (WB)

(Western blot analysis of MyD88 Antibody (Center) in HepG2 cell line lysates (35ug/lane). MyD88 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MyD88 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MyD88 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-MyD88 antibody

Antibodies; Adapter Proteins

References

1. Bannon, C., et.al., Biochem. J. 423(1), 119-128 (2009). 2. Burns, K., et.al., J. Biol. Chem. 273(20), 12203-12209 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 197276654
• NCBI GeneID: 4615
• NCBI Accession #: NP_002459.2
• NCBI GenBank Nucleotide #: NM_002468.4
• UniProt Accession #: Q99836; P78397; Q53XS7; B4DKH8; B4DKU4; B4DQ60; B4DQ72; J3KPU4; J3KQ87; J3KQJ6
• Molecular Weight: 34,097 Da
• NCBI Official Full Name: myeloid differentiation primary response protein MyD88 isoform 2
• NCBI Official Synonym Full Names: myeloid differentiation primary response 88
• NCBI Official Symbol: MYD88
• NCBI Official Synonym Symbols: MYD88D
• NCBI Protein Information: myeloid differentiation primary response protein MyD88; myeloid differentiation primary response gene (88)
• UniProt Protein Name: Myeloid differentiation primary response protein MyD88
• Protein Family: Myeloid differentiation primary response protein
• UniProt Gene Name: MYD88
• UniProt Entry Name: MYD88_HUMAN

NCBI Description

This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]