Dot Blot (DB)
(Dot blot analysis of Phospho-MYC-T58 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Rabbit anti-Human MYC Polyclonal Antibody
MYC, phosphorylated (Thr58) (Myc Proto-oncogene Protein, c-Myc, Proto-oncogene c-Myc, Transcription Factor p64, Class E Basic Helix-loop-helix Protein 39, bHLHe39)
Reactivity
Human
Applications
ELISA, Western Blot, Gel Super Shift Assay
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
MYC; Polyclonal Antibody; phosphorylated (Thr58) (Myc Proto-oncogene Protein; c-Myc; Proto-oncogene c-Myc; Transcription Factor p64; Class E Basic Helix-loop-helix Protein 39; bHLHe39); Anti -MYC; anti-MYC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human MYC when phosphorylated at Thr58.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Sequence Length
425
Applicable Applications for anti-MYC antibody
ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in ELISA, Western Blot, and Dot Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:200
Dot Blot: 1:100-1:500
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:200
Dot Blot: 1:100-1:500
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr58 of human MYC (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Dot Blot (DB)
(Dot blot analysis of Phospho-MYC-T58 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Dot Blot (DB)
(Dot blot analysis of Phospho-MYC-T58 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Western Blot (WB)
(Western blot analysis of Phospho-MYC-T58 Antibody in human TPA activated Hela cell line lysates. Phospho-MYC (arrow) was detected using the purified PAb. (0: without TPA; 1: 60ug/ml TPA, 15min; 2: 60ug/ml TPA, 30min; 3: 60ug/ml TPA, 45min; 4: 125ug/ml TPA, 15min; 5: 125ug/ml TPA, 30min; 6: 125ug/ml TPA, 45min; 7: 250ug/ml TPA, 15min; 8: 250ug/ml TPA, 30min; 9: 250ug/ml, 45min))
Western Blot (WB)
(Western blot analysis of Phospho-MYC-T58 Antibody in human TPA activated Hela cell line lysates. Phospho-MYC (arrow) was detected using the purified PAb. (0: without TPA; 1: 60ug/ml TPA, 15min; 2: 60ug/ml TPA, 30min; 3: 60ug/ml TPA, 45min; 4: 125ug/ml TPA, 15min; 5: 125ug/ml TPA, 30min; 6: 125ug/ml TPA, 45min; 7: 250ug/ml TPA, 15min; 8: 250ug/ml TPA, 30min; 9: 250ug/ml, 45min))
Related Product Information for anti-MYC antibody
MYC participates in the regulation of gene transcription. It binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. This protein appears to activate the transcription of growth-related genes. Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia.
Product Categories/Family for anti-MYC antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
MYC
Protein Family
Similar Products
Product Notes
The MYC (Catalog #AAA629186) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYC, phosphorylated (Thr58) (Myc Proto-oncogene Protein, c-Myc, Proto-oncogene c-Myc, Transcription Factor p64, Class E Basic Helix-loop-helix Protein 39, bHLHe39) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYC can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA). Suitable for use in ELISA, Western Blot, and Dot Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:200 Dot Blot: 1:100-1:500. Researchers should empirically determine the suitability of the MYC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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