Rabbit anti-Human MYBBP1A Polyclonal Antibody | anti-MYBBP1A antibody

MYBBP1A, NT (MYBBP1A, P160, Myb-binding protein 1A) (APC)

Cat. Num. AAA6323122

• Gene Names: MYBBP1A; P160; PAP2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MYBBP1A; Polyclonal Antibody; NT (MYBBP1A; P160; Myb-binding protein 1A) (APC); Myb-binding protein 1A; anti-MYBBP1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MYBBP1A antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MYBBP1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-287 amino acids from the N-terminal region of human MYBBP1A.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MYBBP1A Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MYBBP1A antibody detected the MYBBP1A protein (arrow).)

Related Product Information for anti-MYBBP1A antibody

MYBBP1A may activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity) (By similarity).

Product Categories/Family for anti-MYBBP1A antibody

Antibodies; Binding Proteins

References

Perrera, C., et al. J. Biol. Chem. 285(16):11775-11785(2010). Fuller, H.R., et al. J. Proteome Res. 9(1):556-563(2010). Redmond, A.M., et al. Clin. Cancer Res. 15(6):2098-2106(2009). Lu, Y., et al. J. Lipid Res. 49(12):2582-2589(2008). Al-azawi, D., et al. Oncogene 27(21):3021-3031(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 157694492
• NCBI GeneID: 10514
• NCBI Accession #: NP_055335.2
• NCBI GenBank Nucleotide #: NM_014520.3
• UniProt Accession #: Q9BQG0; Q86VM3; Q9BW49; Q9P0V5; Q9UF99
• Molecular Weight: 148,855 Da
• NCBI Official Full Name: myb-binding protein 1A isoform 2
• NCBI Official Synonym Full Names: MYB binding protein (P160) 1a
• NCBI Official Symbol: MYBBP1A
• NCBI Official Synonym Symbols: P160; PAP2
• NCBI Protein Information: myb-binding protein 1A; p53-activated protein-2
• UniProt Protein Name: Myb-binding protein 1A
• Protein Family: Myb-binding protein
• UniProt Gene Name: MYBBP1A
• UniProt Synonym Gene Names: P160
• UniProt Entry Name: MBB1A_HUMAN

NCBI Description

This gene encodes a nucleolar transcriptional regulator that was first identified by its ability to bind specifically to the Myb proto-oncogene protein. The encoded protein is thought to play a role in many cellular processes including response to nucleolar stress, tumor suppression and synthesis of ribosomal DNA. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

MYBBP1A: may activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity). Binds to and represses JUN and MYB via the leucine zipper regions present in these proteins. Also binds to and represses PGC-1 (PPAR gamma coactivator-1): this interaction is abrogated when PGC-1 is phosphorylated by ERK1. Binds to and stimulates transcription by AhR. Binds to KPNA2. Shuttles between the nucleus and cytoplasm. Nuclear import may be mediated by KPNA2, while export appears to depend partially on exportin 1. Predominantly nucleolar. Two alternatively spliced isoforms have been described.

Protein type: Nucleolus; DNA replication; DNA-binding; RNA-binding

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: NLS-dependent protein nuclear import complex; intracellular membrane-bound organelle; membrane; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; DNA-directed DNA polymerase activity; transcription corepressor activity; transcription factor binding

Biological Process: osteoblast differentiation; cellular response to glucose starvation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; nucleocytoplasmic transport; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent