Rabbit anti-Human MYB Polyclonal Antibody | anti-MYB antibody

MYB, ID (MYB, Transcriptional activator Myb, Proto-oncogene c-Myb) (FITC)

Cat. Num. AAA6323113

• Gene Names: MYB; efg; Cmyb; c-myb; c-myb_CDS
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MYB; Polyclonal Antibody; ID (MYB; Transcriptional activator Myb; Proto-oncogene c-Myb) (FITC); Proto-oncogene c-Myb; anti-MYB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MYB antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MYB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 418-448 amino acids from the Central region of human MYB.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MYB Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MYB antibody detected the MYB protein (arrow).)

Immunohistochemistry (IHC)

(MYB Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MYB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-MYB antibody

This gene encodes a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-MYB antibody

Antibodies; Transcription Factors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Tanno, B., et al. J. Biol. Chem. 285(38):29434-29445(2010). Melen, E., et al. J. Allergy Clin. Immunol. 126(3):631-637(2010). Stenman, G., et al. Cell Cycle 9(15):2986-2995(2010). Deisenroth, C., et al. Mol. Cell. Biol. 30(16):3981-3993(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 194328729
• NCBI GeneID: 4602
• NCBI Accession #: NP_001123645.1; NP_001123644.1; NP_005366.2
• NCBI GenBank Nucleotide #: NM_001130173.1
• UniProt Accession #: P10242; P78391; P78392; P78525; P78526; Q14023; Q14024; E9PI07; E9PLZ5; E9PNA4; E9PNL6; E9PRS2
• Molecular Weight: 37,507 Da
• NCBI Official Full Name: transcriptional activator Myb isoform 1
• NCBI Official Synonym Full Names: MYB proto-oncogene, transcription factor
• NCBI Official Symbol: MYB
• NCBI Official Synonym Symbols: efg; Cmyb; c-myb; c-myb_CDS
• NCBI Protein Information: transcriptional activator Myb
• UniProt Protein Name: Transcriptional activator Myb
• Protein Family: Myb protein
• UniProt Gene Name: MYB
• UniProt Entry Name: MYB_HUMAN

NCBI Description

This gene encodes a protein with three HTH DNA-binding domains that functions as a transcription regulator. This protein plays an essential role in the regulation of hematopoiesis. This gene may be aberrently expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

Myb: Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 6q22-q23

Cellular Component: nuclear matrix; cytosol

Molecular Function: protein binding; chromatin binding

Biological Process: positive regulation of histone H3-K9 methylation; thymus development; transcription, DNA-dependent; in utero embryonic development; positive regulation of transcription, DNA-dependent; homeostasis of number of cells; positive regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; chromatin remodeling; positive regulation of T-helper cell differentiation; regulation of transcription, DNA-dependent; calcium ion transport; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; blood coagulation; embryonic gut development; G1/S transition of mitotic cell cycle