Rabbit anti-Human MuSK Polyclonal Antibody | anti-MuSK antibody

MuSK, CT (Muscle Skeletal Receptor Tyrosine Kinase, MGC126323, MGC126324, Muscle Specific Kinase Receptor, Muscle Specific Tyrosine Protein Kinase Receptor, Receptor Tyrosine Kinase MuSK, Skeletal Muscle Receptor Tyrosine Kinase) (Biotin)

Cat. Num. AAA6488689

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MuSK; Polyclonal Antibody; CT (Muscle Skeletal Receptor Tyrosine Kinase; MGC126323; MGC126324; Muscle Specific Kinase Receptor; Muscle Specific Tyrosine Protein Kinase Receptor; Receptor Tyrosine Kinase MuSK; Skeletal Muscle Receptor Tyrosine Kinase) (Biotin); EC=2.7.10.1; MDK4; Muscle/Skeletal Receptor Tyrosine Protein Kinase; Neural Fold Somite Kinase 1; Nsk1; Neural Fold Somite Kinase 2; Nsk2; Neural Fold Somite Kinase 3; Nsk3; anti-MuSK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MUSK.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MuSK antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MUSK (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry (FC/FACS)

(MUSK Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of anti-MUSK Pab in placenta cell lysate. MUSK (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-MuSK antibody

Antibodies; Receptors; Tyrosine Kinase

References

1. Blume-Jensen P, et al. Nature 2001. 411: 355. 2. Cantrell D, J. Cell Sci. 2001. 114: 1439. 3. Jhiang S Oncogene 2000. 19: 5590. 4. Manning G, et al. Science 2002. 298: 1912. 5. Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359. 6.Robertson, S. et al. Trends Genet. 2000. 16: 368. 7.Robinson D, et al. Oncogene 2000. 19: 5548. 8.Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889. 9.Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561. 10.Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.

NCBI and Uniprot Product Information

• NCBI GI #: 261878455
• NCBI GeneID: 4593
• NCBI Accession #: NP_001159752.1
• NCBI GenBank Nucleotide #: NM_001166280.1
• UniProt Accession #: O15146; Q32MJ8; Q32MJ9; Q5VZW7; Q5VZW8
• Molecular Weight: 97,056 Da
• NCBI Official Full Name: muscle, skeletal receptor tyrosine-protein kinase isoform 2
• NCBI Official Synonym Full Names: muscle, skeletal, receptor tyrosine kinase
• NCBI Official Symbol: MUSK
• NCBI Protein Information: muscle, skeletal receptor tyrosine-protein kinase; muscle-specific kinase receptor; muscle-specific tyrosine-protein kinase receptor
• UniProt Protein Name: Muscle, skeletal receptor tyrosine-protein kinase
• Protein Family: Muskelin
• UniProt Gene Name: MUSK
• UniProt Synonym Gene Names: MuSK
• UniProt Entry Name: MUSK_HUMAN

NCBI Description

This gene encodes a muscle-specific tyrosine kinase receptor. The encoded protein may play a role in clustering of the acetylcholine receptor in the postsynaptic neuromuscular junction. Mutations in this gene have been associated with congenital myasthenic syndrome. Alternatively spliced transcript variants have been described.[provided by RefSeq, Oct 2009]

Uniprot Description

MUSK: a receptor tyrosine kinase that is essential for the establishment and maintenance of the neuromuscular junction (NMJ). Its activation by agrin, a neuronally derived heparan-sulfate proteoglycan, and the agrin receptor (LRP4), leads to clustering of acetylcholine receptors on the postsynaptic side of the NMJ. Its activation by agrin requires Dok7, which interacts with the cytoplasmic portion of MuSK and activates its tyrosine kinase activity.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; Membrane protein, integral; TK group; Musk family

Chromosomal Location of Human Ortholog: 9q31.3-q32

Cellular Component: postsynaptic membrane; integral to plasma membrane; cell junction; neuromuscular junction; receptor complex

Molecular Function: protein binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: extracellular matrix organization and biogenesis; peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; positive regulation of neuron apoptosis; multicellular organismal development; regulation of synaptic growth at neuromuscular junction; positive regulation of protein amino acid phosphorylation; cell differentiation; memory; neuromuscular junction development; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Myasthenic Syndrome, Congenital, 9, Associated With Acetylcholine Receptor Deficiency; Fetal Akinesia Deformation Sequence