Rabbit anti-Human Munc13-2 Polyclonal Antibody | anti-Munc13-2 antibody

Munc13-2, ID (UNC13B, munc13, Protein unc-13 Homolog B, UNC13) (Biotin)

Cat. Num. AAA6486420

• Gene Names: UNC13B; UNC13; MUNC13; Unc13h2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Munc13-2; Polyclonal Antibody; ID (UNC13B; munc13; Protein unc-13 Homolog B; UNC13) (Biotin); anti-Munc13-2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UNC13B.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-Munc13-2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human UNC13B (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of UNC13B Antibody (Center) in 293 cell line lysates (35ug/lane). UNC13B (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(UNC13B Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-Munc13-2 antibody

Antibodies; Proteins

References

1. Sjoeblom T., et.al., Science 314:268-274(2006). 2. Yu L.-R., Zhu Z., et.al., J. Proteome Res. 6:4150-4162(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 110611226
• NCBI GeneID: 10497
• NCBI Accession #: NP_006368.3
• NCBI GenBank Nucleotide #: NM_006377.3
• UniProt Accession #: O14795; Q5VYM8
• Molecular Weight: 180,679 Da
• NCBI Official Full Name: protein unc-13 homolog B
• NCBI Official Synonym Full Names: unc-13 homolog B (C. elegans)
• NCBI Official Symbol: UNC13B
• NCBI Official Synonym Symbols: UNC13; MUNC13; Unc13h2
• NCBI Protein Information: protein unc-13 homolog B; munc13-2; homolog of rat Munc13 (diacylglycerol-binding)
• UniProt Protein Name: Protein unc-13 homolog B
• UniProt Gene Name: UNC13B
• UniProt Synonym Gene Names: UNC13; munc13
• UniProt Entry Name: UN13B_HUMAN

Uniprot Description

UNC13B: Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity- depending refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. Belongs to the unc-13 family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: Golgi apparatus; cytoplasm; presynaptic active zone; plasma membrane; terminal button; cell junction; cytosol; neuromuscular junction

Molecular Function: diacylglycerol binding; protein binding; signal transducer activity; metal ion binding; receptor activity

Biological Process: synaptic transmission, glutamatergic; synaptic transmission; glutamate secretion; apoptosis; neurotransmitter secretion; regulation of short-term neuronal synaptic plasticity; excretion; signal transduction; synaptic vesicle priming; neuromuscular junction development; synaptic vesicle docking during exocytosis