Rabbit anti-Human MUC1 Polyclonal Antibody | anti-MUC1 antibody

MUC1-T1224 (MUC1, PUM, Mucin-1, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen,

Cat. Num. AAA6322889

• Gene Names: MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MUC1; Polyclonal Antibody; MUC1-T1224 (MUC1; PUM; Mucin-1; Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; PEMT; Peanut-reactive urinary mucin; Polymorphic epithelial mucin; Tumor-associated epithelial membrane antigen; ; Tumor-associated mucin; CD227; Mucin-1 subunit alpha; Mucin-1 subunit beta; MUC1-CT; anti-MUC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MUC1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MUC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1209-1239 amino acids from human MUC1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MUC1-T1224 Antibody western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MUC1 antibody detected the MUC1 protein (arrow).)

Related Product Information for anti-MUC1 antibody

This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined. [provided by RefSeq].

Product Categories/Family for anti-MUC1 antibody

Antibodies; CD Markers

References

Behrens, M.E., et al. Oncogene 29(42):5667-5677(2010). Lacunza, E., et al. Cancer Genet. Cytogenet. 201(2):102-110(2010). Meyer, T.E., et al. PLoS Genet. 6 (8) (2010): Beatson, R.E., et al. Immunotherapy 2(3):305-327(2010). Caffery, B., et al. Mol. Vis. 16, 1720-1727 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 113206026
• NCBI GeneID: 4582
• NCBI Accession #: NP_001037856.1; NP_001037858.1; NP_002447.4; NP_001037855.1; NP_001018016.1; NP_001037857.1
• NCBI GenBank Nucleotide #: NM_001044391.2
• UniProt Accession #: P15941; P13931; P15942; P17626; A5YRV1; A6ZID9; A6ZIE0; B1AVQ8; B1AVR0; B6ECA1; E7ESE5; E7EUG9
• Molecular Weight: 28,297 Da
• NCBI Official Full Name: mucin-1 isoform 6
• NCBI Official Synonym Full Names: mucin 1, cell surface associated
• NCBI Official Symbol: MUC1
• NCBI Official Synonym Symbols: EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
• NCBI Protein Information: mucin-1
• UniProt Protein Name: Mucin-1
• Protein Family: Mucin
• UniProt Gene Name: MUC1
• UniProt Synonym Gene Names: PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta
• UniProt Entry Name: MUC1_HUMAN

NCBI Description

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]

Uniprot Description

MUC1: a large cell surface glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. Plays a role in adhesion and cell-cell interactions, metastasis and signaling. May provide a protective layer on epithelial surfaces. Direct or indirect interaction with actin cytoskeleton. Its cytoplasmic tail (MUC1CT) is involved in several signaling pathways, including those involving Ras, beta-catenin, p120 catenin, p53 and estrogen receptor alpha. MUC1CT also forms complexes with transcription factors, and then translocates to the nucleus by an unknown mechanism, where it is believed to influence the transcription of their target genes. MUC1CT has also been proposed to localize to mitochondrial membranes under conditions of genotoxic stress, where it attenuates the apoptotic pathway in response and confers resistance to apoptosis-inducing drugs. Aberrantly glycosylated forms expressed in human epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Nine alternatively spliced isoforms have been described. Isoforms 5 and 9 are secreted. Isoform 7, expressed only in tumor cells, is a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling. Can bind to GRB2 adapter protein.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Nuclear receptor co-regulator; Tumor suppressor; Cell adhesion; Actin-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; cell surface; integral to plasma membrane; Golgi lumen; apical plasma membrane; nuclear chromatin; cytoplasm; vesicle

Molecular Function: protein binding; p53 binding; transcription cofactor activity

Biological Process: protein amino acid O-linked glycosylation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; regulation of transcription from RNA polymerase II promoter in response to stress; cellular protein metabolic process; O-glycan processing; post-translational protein modification; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest

Disease: Medullary Cystic Kidney Disease 1