Rabbit anti-Human MTHFD1 Polyclonal Antibody | anti-MTHFD1 antibody

MTHFD1, ID (P550) (C-1-tetrahydrofolate Synthase, Cytoplasmic, C1-THF Synthase, Methylenetetrahydrofolate Dehydrogenase, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase, MTHFC, MTHFD) (HRP)

Cat. Num. AAA6487925

• Gene Names: MTHFD1; MTHFC; MTHFD
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: MTHFD1; Polyclonal Antibody; ID (P550) (C-1-tetrahydrofolate Synthase; Cytoplasmic; C1-THF Synthase; Methylenetetrahydrofolate Dehydrogenase; Methenyltetrahydrofolate Cyclohydrolase; Formyltetrahydrofolate Synthetase; MTHFC; MTHFD) (HRP); anti-MTHFD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MTHFD1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MTHFD1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the Center (P550) region of human MTHFD1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MTHFD1 Antibody (Center P550) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Western Blot (WB)

(Western blot analysis of MTHFD1 Antibody (Center P550) in Y79 cell line lysates (35ug/lane). MTHFD1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(MTHFD1 Antibody (Center P550) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MTHFD1 Antibody (Center P550) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-MTHFD1 antibody

Antibodies; Proteins

References

1. Ivanov, A., J Am Diet Assoc 109(2), 313-318 (2009). 2. Schmidt A., Biochemistry 39:6325-6335(2000). 3. Hol F.A., Clin. Genet. 53:119-125(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 222136639
• NCBI GeneID: 4522
• NCBI Accession #: NP_005947
• NCBI GenBank Nucleotide #: NM_005956
• UniProt Accession #: P11586; Q86VC9; Q9BVP5; B2R5Y2; G3V2B8
• Molecular Weight: 101,559 Da
• NCBI Official Full Name: C-1-tetrahydrofolate synthase, cytoplasmic
• NCBI Official Synonym Full Names: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
• NCBI Official Symbol: MTHFD1
• NCBI Official Synonym Symbols: MTHFC; MTHFD
• NCBI Protein Information: C-1-tetrahydrofolate synthase, cytoplasmic; 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase; C1-THF synthase; cytoplasmic C-1-tetrahydrofolate synthase
• UniProt Protein Name: C-1-tetrahydrofolate synthase, cytoplasmic
• UniProt Gene Name: MTHFD1
• UniProt Synonym Gene Names: MTHFC; MTHFD; C1-THF synthase
• UniProt Entry Name: C1TC_HUMAN

Uniprot Description

MTHFD1: an ubiquitous trifunctional enzyme containing two major domains: a N-terminal part, containing methylene-THF dehydrogenase and methenyl-THF cyclohydrolase activities, and a C-terminal part containing a formyl-THF synthetase domain.

Protein type: EC 1.5.1.5; Oxidoreductase; Ligase; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 6.3.4.3; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Hydrolase; EC 3.5.4.9

Chromosomal Location of Human Ortholog: 14q24

Cellular Component: mitochondrion; membrane; cytosol

Molecular Function: methylenetetrahydrofolate dehydrogenase (NADP+) activity; formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity; ATP binding; methylenetetrahydrofolate dehydrogenase activity

Biological Process: histidine biosynthetic process; folic acid and derivative biosynthetic process; vitamin metabolic process; methionine biosynthetic process; purine nucleotide biosynthetic process; folic acid metabolic process; one-carbon compound metabolic process; water-soluble vitamin metabolic process

Disease: Neural Tube Defects, Folate-sensitive