Rabbit anti-Human MTBP Polyclonal Antibody | anti-MTBP antibody

MTBP, CT (Mdm2-binding Protein, hMTBP) (MaxLight 750)

Cat. Num. AAA6488674

• Gene Names: MTBP; MDM2BP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: MTBP; Polyclonal Antibody; CT (Mdm2-binding Protein; hMTBP) (MaxLight 750); anti-MTBP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MTBP.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-MTBP antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MTBP (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MTBP antibody (C-term) in K562 cell line lysates (35ug/lane). MTBP (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with MTBP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of K562 cells using MTBP Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-MTBP antibody

Antibodies; Apoptosis

References

1. Brady, M., Mol. Cell. Biol. 25(2), 545-553 (2005). 2. Boyd, M.T., J. Biol. Chem. 275(41), 31883-31890 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 21630257
• NCBI GeneID: 27085
• NCBI Accession #: NP_071328
• NCBI GenBank Nucleotide #: NM_022045.4
• UniProt Accession #: Q96DY7; Q9HA89; B4DUR5
• Molecular Weight: 104 kDa
• NCBI Official Full Name: mdm2-binding protein
• NCBI Official Synonym Full Names: MDM2 binding protein
• NCBI Official Symbol: MTBP
• NCBI Official Synonym Symbols: MDM2BP
• NCBI Protein Information: mdm2-binding protein; MDM2 (mouse double minute 2)-binding protein, 104kD; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein binding protein, 104kDa
• UniProt Protein Name: Mdm2-binding protein
• Protein Family: Mdm2-binding protein
• UniProt Gene Name: MTBP
• UniProt Synonym Gene Names: hMTBP
• UniProt Entry Name: MTBP_HUMAN

NCBI Description

This gene encodes a protein that interacts with the oncoprotein mouse double minute 2. The encoded protein regulates progression through the cell cycle and may be involved in tumor formation. [provided by RefSeq, Aug 2012]

Uniprot Description

MTBP: Inhibits cell migration in vitro and suppresses the invasive behavior of tumor cells. May play a role in MDM2-dependent p53/TP53 homeostasis in unstressed cells. Inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of p53/TP53 and its subsequent degradation. Belongs to the MTBP family.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8q24.12

Cellular Component: kinetochore; chromatin

Biological Process: negative regulation of cell proliferation; traversing start control point of mitotic cell cycle; negative regulation of mitosis; cell cycle arrest