Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with MSX1 Antibody (Center).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MSX1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).MSX1 immunoreactivity is localized to Nucleus significantly. )
Rabbit anti-Human, Mouse MSX1 Polyclonal Antibody | anti-MSX1 antibody
MSX1, ID (MSX1, HOX7, Homeobox protein MSX-1, Homeobox protein Hox-7, Msh homeobox 1-like protein) (PE)
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with MSX1 Antibody (Center).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MSX1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).MSX1 immunoreactivity is localized to Nucleus significantly. )
Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with MSX1 Antibody (Center).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MSX1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).MSX1 immunoreactivity is localized to Nucleus significantly. )
Western Blot (WB)
(MSX1 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).)
Western Blot (WB)
(MSX1 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).)
Western Blot (WB)
(MSX1 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).)
Western Blot (WB)
(MSX1 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).)
Immunofluorescence (IF)
(Fluorescent confocal image of SY5Y cells stained with MSX1 (Center) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP19261c MSX1 (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the MSX1 mainly to the nucleus.)
Immunofluorescence (IF)
(Fluorescent confocal image of SY5Y cells stained with MSX1 (Center) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP19261c MSX1 (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the MSX1 mainly to the nucleus.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the muscle segment homeobox gene family. The encoded protein functions as a transcriptional repressor during embryogenesis through interactions with components of the core transcription complex and other homeoproteins. It may also have roles in limb-pattern formation, craniofacial development, particularly odontogenesis, and tumor growth inhibition. Mutations in this gene, which was once known as homeobox 7, have been associated with nonsyndromic cleft lip with or without cleft palate 5, Witkop syndrome, Wolf-Hirschom syndrome, and autosomoal dominant hypodontia. [provided by RefSeq, Jul 2008]
Uniprot Description
MSX1: Acts as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis. Expression in the developing nail bed mesenchyme is important for nail plate thickness and integrity. Expressed in the developing nail bed mesenchyme. Belongs to the Msh homeobox family.
Protein type: Motility/polarity/chemotaxis; DNA-binding
Chromosomal Location of Human Ortholog: 4p16.2
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus
Molecular Function: p53 binding
Biological Process: embryonic forelimb morphogenesis; negative regulation of striated muscle cell differentiation; transcription from RNA polymerase II promoter; muscle development; protein stabilization; in utero embryonic development; regulation of odontogenesis; mesenchymal cell proliferation; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; palate development; middle ear morphogenesis; embryonic hindlimb morphogenesis; odontogenesis of dentine-containing teeth; anterior/posterior pattern formation; negative regulation of cell proliferation; pituitary gland development; midbrain development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of cell growth; positive regulation of BMP signaling pathway; negative regulation of apoptosis
Disease: Orofacial Cleft 5; Witkop Syndrome; Tooth Agenesis, Selective, 1
Research Articles on MSX1
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Product Notes
The MSX1 msx1 (Catalog #AAA6322625) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MSX1, ID (MSX1, HOX7, Homeobox protein MSX-1, Homeobox protein Hox-7, Msh homeobox 1-like protein) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MSX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MSX1 msx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MSX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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