Rabbit anti-Human, Mouse MSH2 Polyclonal Antibody | anti-MSH2 antibody

MSH2 Antibody

Cat. Num. AAA7103535

• Gene Names: MSH2; FCC1; COCA1; HNPCC; LCFS2; HNPCC1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: >95%, Protein G purified
• Synonyms: MSH2; Polyclonal Antibody; MSH2 Antibody; DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2; anti-MSH2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 934

• Applicable Applications for anti-MSH2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:5000; IHC: 1:200-1:500; IP: 1:200-1:2000
• Immunogen: Recombinant Human DNA mismatch repair protein Msh2 protein (2-254AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysateAll lanes: MSH2 antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 105, 98 KDaObserved band size: 105, 98 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7103535 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunoprecipitation (IP)

(Immunoprecipitating MSH2 in Hela whole cell lysateLane 1: Rabbit control IgG (1ug)instead of MBS7103535 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7103535 (6ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))

Related Product Information for anti-MSH2 antibody

Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Product Categories/Family for anti-MSH2 antibody

Epigenetics and Nuclear Signaling; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4557761
• NCBI GeneID: 4436
• NCBI Accession #: NP_000242.1
• NCBI GenBank Nucleotide #: NM_000251.2
• UniProt Accession #: P43246; O75488; B4E2Z2
• Molecular Weight: 97,323 Da
• NCBI Official Full Name: DNA mismatch repair protein Msh2 isoform 1
• NCBI Official Synonym Full Names: mutS homolog 2
• NCBI Official Symbol: MSH2
• NCBI Official Synonym Symbols: FCC1; COCA1; HNPCC; LCFS2; HNPCC1
• NCBI Protein Information: DNA mismatch repair protein Msh2
• UniProt Protein Name: DNA mismatch repair protein Msh2
• Protein Family: DNA mismatch repair protein
• UniProt Gene Name: MSH2
• UniProt Synonym Gene Names: hMSH2

NCBI Description

This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.