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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MRPS30 antibody.)

Rabbit anti-Human, Mouse MRPS30 Polyclonal Antibody | anti-MRPS30 antibody

MRPS30 (Ribosomal Protein S30, Mitochondrial, PAP, PDCD9, S30mt, MRP-S30) (FITC)

Gene Names
MRPS30; PAP; PDCD9; S30mt; MRP-S30
Reactivity
Human, Mouse
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified by affinity chromatography.
Synonyms
MRPS30; Polyclonal Antibody; MRPS30 (Ribosomal Protein S30; Mitochondrial; PAP; PDCD9; S30mt; MRP-S30) (FITC); anti-MRPS30 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes MRPS30. Species Crossreactivity: human, mouse
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-MRPS30 antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
IF: 1:50-1:200
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human MRPS30.
Conjugate
FITC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MRPS30 antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MRPS30 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using MRPS30 antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cell using MRPS30 antibody. Blue: DAPI for nuclear staining.)
Product Categories/Family for anti-MRPS30 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,365 Da
NCBI Official Full Name
28S ribosomal protein S30, mitochondrial
NCBI Official Synonym Full Names
mitochondrial ribosomal protein S30
NCBI Official Symbol
MRPS30
NCBI Official Synonym Symbols
PAP; PDCD9; S30mt; MRP-S30
NCBI Protein Information
28S ribosomal protein S30, mitochondrial; programmed cell death 9
UniProt Protein Name
28S ribosomal protein S30, mitochondrial
Protein Family
UniProt Gene Name
MRPS30
UniProt Synonym Gene Names
PDCD9; MRP-S30; S30mt
UniProt Entry Name
RT30_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPS30: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available. [provided by RefSeq, Jul 2008]

Chromosomal Location of Human Ortholog: 5q11

Cellular Component: mitochondrial inner membrane; ribosome

Molecular Function: structural constituent of ribosome

Biological Process: mitochondrial translation; apoptosis; organelle organization and biogenesis

Research Articles on MRPS30

Similar Products

Product Notes

The MRPS30 mrps30 (Catalog #AAA6427423) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MRPS30 (Ribosomal Protein S30, Mitochondrial, PAP, PDCD9, S30mt, MRP-S30) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MRPS30 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC). IF: 1:50-1:200 WB: 1:500-1:2000 IHC: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MRPS30 mrps30 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRPS30, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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