Western Blot (WB)
(MRPS15 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MRPS15 antibody detected the MRPS15 protein (arrow).)
Rabbit anti-Human MRPS15 Polyclonal Antibody | anti-MRPS15 antibody
MRPS15, ID (MRPS15, RPMS15, 28S ribosomal protein S15, mitochondrial) (Azide free) (HRP)
Gene Names
MRPS15; DC37; S15mt; RPMS15; MPR-S15
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MRPS15; Polyclonal Antibody; ID (MRPS15; RPMS15; 28S ribosomal protein S15; mitochondrial) (Azide free) (HRP); mitochondrial; anti-MRPS15 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-MRPS15 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
MRPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-153 amino acids from the Central region of human MRPS15.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(MRPS15 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MRPS15 antibody detected the MRPS15 protein (arrow).)
Western Blot (WB)
(MRPS15 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MRPS15 antibody detected the MRPS15 protein (arrow).)
Related Product Information for anti-MRPS15 antibody
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.
Product Categories/Family for anti-MRPS15 antibody
References
Zhang, Z., et al. Genomics 81(5):468-480(2003). Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001): Suzuki, T., et al. J. Biol. Chem. 276(35):33181-33195(2001). Cavdar Koc, E., et al. J. Biol. Chem. 276(22):19363-19374(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,842 Da
NCBI Official Full Name
28S ribosomal protein S15, mitochondrial
NCBI Official Synonym Full Names
mitochondrial ribosomal protein S15
NCBI Official Symbol
MRPS15
NCBI Official Synonym Symbols
DC37; S15mt; RPMS15; MPR-S15
NCBI Protein Information
28S ribosomal protein S15, mitochondrial; MRP-S15
UniProt Protein Name
28S ribosomal protein S15, mitochondrial
Protein Family
UniProt Gene Name
MRPS15
UniProt Synonym Gene Names
RPMS15; DC37; MRP-S15; S15mt
UniProt Entry Name
RT15_HUMAN
Similar Products
Product Notes
The MRPS15 mrps15 (Catalog #AAA6322300) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MRPS15, ID (MRPS15, RPMS15, 28S ribosomal protein S15, mitochondrial) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MRPS15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MRPS15 mrps15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRPS15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
