Rabbit anti-Mouse, Human MRPL54 Polyclonal Antibody | anti-MRPL54 antibody

Anti-MRPL54 Antibody (aa81-130) IHC-plus

Cat. Num. AAA249836

• Gene Names: MRPL54; L54mt; MRP-L54
• Reactivity: Mouse, Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity purified
• Synonyms: MRPL54; Polyclonal Antibody; Anti-MRPL54 Antibody (aa81-130) IHC-plus; Rabbit Polyclonal (IgG) to Human MRPL54; Human MRPL54; L54mt; MRP-L54; anti-MRPL54 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MRPL54 Antibody detects endogenous levels of total MRPL54 protein.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 138

• Applicable Applications for anti-MRPL54 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:40000); IHC-P (10 ug/ml); WB (1:500 - 1:1000)
• Immunogen: MRPL54 antibody was raised against synthetic peptide from human MRPL54 (aa81-130).
• Immunogen Description: Synthetic peptide from human MRPL54 (aa81-130).
• Antigen Modification: aa81-130
• Target Species: Human
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-MRPL54 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts from Jurkat cells, using MRPL54 Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-MRPL54 antibody

MRPL54 Antibody, L54mt Antibody, MRP-L54 Antibody Description: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA.

NCBI and Uniprot Product Information

• NCBI GI #: 74737109
• NCBI GeneID: 116541
• UniProt Accession #: Q6P161
• Molecular Weight: 15,819 Da
• NCBI Official Full Name: 39S ribosomal protein L54, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L54
• NCBI Official Symbol: MRPL54
• NCBI Official Synonym Symbols: L54mt; MRP-L54
• NCBI Protein Information: 39S ribosomal protein L54, mitochondrial
• UniProt Protein Name: 39S ribosomal protein L54, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL54
• UniProt Synonym Gene Names: L54mt; MRP-L54
• UniProt Entry Name: RM54_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPL54:

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: mitochondrial inner membrane; ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis