Rabbit anti-Human, Mouse MRPL24 Polyclonal Antibody | anti-MRPL24 antibody

MRPL24, CT (39S Ribosomal Protein L24, Mitochondrial, L24mt, MRP-L24) (PE)

Cat. Num. AAA6488598

• Gene Names: MRPL24; L24mt; MRP-L18; MRP-L24
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MRPL24; Polyclonal Antibody; CT (39S Ribosomal Protein L24; Mitochondrial; L24mt; MRP-L24) (PE); anti-MRPL24 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MRPL24. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MRPL24 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MRPL24 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-MRPL24 Pab in mouse kidney tissue lysate (35ug/lane). MRPL24 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-MRPL24 antibody

Antibodies; Ribosomal Proteins

References

1. Zhang, Z., et al., Genomics 81(5):468-480 (2003). 2. O'Brien, T.W., et al., J. Biol. Chem. 275(24):18153-18159 (2000). 3. Kenmochi, N., et al., Genomics 77(1-2), 65-70 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 22035588
• NCBI GeneID: 79590
• NCBI Accession #: NP_078816
• NCBI GenBank Nucleotide #: NM_024540
• UniProt Accession #: Q96A35
• Molecular Weight: 25kDa
• NCBI Official Full Name: 39S ribosomal protein L24, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L24
• NCBI Official Symbol: MRPL24
• NCBI Official Synonym Symbols: L24mt; MRP-L18; MRP-L24
• NCBI Protein Information: 39S ribosomal protein L24, mitochondrial
• UniProt Protein Name: 39S ribosomal protein L24, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL24
• UniProt Synonym Gene Names: L24mt; MRP-L24
• UniProt Entry Name: RM24_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which is more than twice the size of its E.coli counterpart (EcoL24). Sequence analysis identified two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPL24: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which is more than twice the size of its E.coli counterpart (EcoL24). Sequence analysis identified two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

Protein type: Mitochondrial; Translation; Ribosomal

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: mitochondrial inner membrane; ribosome

Molecular Function: structural constituent of ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis