Rabbit MRP-S27 Polyclonal Antibody | anti-MRP-S27 antibody

MRP-S27 (Y48) Polyclonal Antibody

Cat. Num. AAA3003639

• Gene Names: MRPS27; S27mt; MRP-S27
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MRP-S27; Polyclonal Antibody; MRP-S27 (Y48) Polyclonal Antibody; MRPS27; mitochondrial ribosomal protein S27; FLJ21764; FLJ23348; KIAA0264; anti-MRP-S27 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MRP-S27 (Y48) polyclonal antibody detects endogenous levels of MRP-S27 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 414

• Applicable Applications for anti-MRP-S27 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 16-62 of Human MRP-S27.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MRP-S27 (Y48) pAb at 1:500 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:BV2 whole cell lysate (20ug) Lane3:C6 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug))

Related Product Information for anti-MRP-S27 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein

NCBI and Uniprot Product Information

• NCBI GI #: 186928850
• NCBI GeneID: 23107
• NCBI Accession #: NP_055899.2
• NCBI GenBank Nucleotide #: NP_055899.2
• UniProt Accession #: Q92552
• Molecular Weight: ~ 47kDa
• NCBI Official Full Name: 28S ribosomal protein S27, mitochondrial isoform 2
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S27
• NCBI Official Symbol: MRPS27
• NCBI Official Synonym Symbols: S27mt; MRP-S27
• NCBI Protein Information: 28S ribosomal protein S27, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S27, mitochondrial
• UniProt Gene Name: MRPS27
• UniProt Synonym Gene Names: KIAA0264; MRP-S27; S27mt
• UniProt Entry Name: RT27_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2013]

Uniprot Description

MRPS27: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.

Protein type: Translation; Ribosomal; Mitochondrial

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: mitochondrial inner membrane; ribosome

Molecular Function: protein binding

Biological Process: mitochondrial translation; organelle organization and biogenesis