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Western Blot (WB) (Western Blot analysis of COLO205 cells using MRP-L21 Polyclonal Antibody)

Rabbit anti-Human, Mouse MRP-L21 Polyclonal Antibody | anti-MRP-L21 antibody

MRP-L21 Polyclonal Antibody

Gene Names
MRPL21; L21mt; MRP-L21
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
MRP-L21; Polyclonal Antibody; MRP-L21 Polyclonal Antibody; MRPL21; 39S ribosomal protein L21; mitochondrial; L21mt; anti-MRP-L21 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
MRP-L21 Polyclonal Antibody detects endogenous levels of MRP-L21 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
205
Applicable Applications for anti-MRP-L21 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:100 - 1:300
ELISA: 1:40000
Not yet tested in other applications.
Immunogen Type
peptide
Immunogen Description
Synthesized peptide derived from the Internal region of human MRP-L21.
Target Name
MRP-L21
Preparation and Storage
Store at -20 degree C/1 year

Western Blot (WB)

(Western Blot analysis of COLO205 cells using MRP-L21 Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of COLO205 cells using MRP-L21 Polyclonal Antibody)
Product Categories/Family for anti-MRP-L21 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,746 Da
NCBI Official Full Name
39S ribosomal protein L21, mitochondrial isoform d
NCBI Official Synonym Full Names
mitochondrial ribosomal protein L21
NCBI Official Symbol
MRPL21
NCBI Official Synonym Symbols
L21mt; MRP-L21
NCBI Protein Information
39S ribosomal protein L21, mitochondrial
UniProt Protein Name
39S ribosomal protein L21, mitochondrial
UniProt Gene Name
MRPL21
UniProt Synonym Gene Names
L21mt; MRP-L21
UniProt Entry Name
RM21_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Multiple transcript variants encoding different isoforms were identified through sequence analysis although some may be subject to nonsense-mediated decay (NMD). [provided by RefSeq, Jul 2008]

Uniprot Description

MRPL21: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Multiple transcript variants encoding different isoforms were identified through sequence analysis although some may be subject to nonsense-mediated decay (NMD). [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Mitochondrial

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: mitochondrial inner membrane; ribosome

Molecular Function: structural constituent of ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis

Research Articles on MRP-L21

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Product Notes

The MRP-L21 mrpl21 (Catalog #AAA9404565) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MRP-L21 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MRP-L21 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:300 ELISA: 1:40000 Not yet tested in other applications. Researchers should empirically determine the suitability of the MRP-L21 mrpl21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRP-L21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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