Rabbit MRE11 Polyclonal Antibody | anti-MRE11 antibody

MRE11 Antibody

Cat. Num. AAA9409339

• Gene Names: MRE11A; ATLD; HNGS1; MRE11; MRE11B
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: MRE11; Polyclonal Antibody; MRE11 Antibody; Meiotic recombination 11 homolog; MRE11A; MRE11B; ATLD; HNGS; anti-MRE11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 708

• Applicable Applications for anti-MRE11 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 14 amino acid peptide from near the amino terminus human MRE11.
• Target Name: MRE11
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of MRE11 in rat lung tissue lysate with MRE11 antibody at (A) 1 and (B) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of MRE11 in rat lung tissue with MRE11 antibody at 5 ug/mL.)

Related Product Information for anti-MRE11 antibody

MRE11 is involved in the repair of DNA double strand breaks as part of a complex that includes the Rad50 and NBS1 protein and is thought to act in the same pathway as the A-T mutated (ATM) protein. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for non-homologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Mutations in this protein result in a novel ataxia telangiectasia-like disorder (ATLD). Unlike the ATM protein, MRE11 is necessary proper mammalian development.

Product Categories/Family for anti-MRE11 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 119587336
• NCBI GeneID: 4361
• Molecular Weight: 81,070 Da
• NCBI Official Full Name: MRE11 meiotic recombination 11 homolog A (S. cerevisiae), isoform CRA_a
• NCBI Official Synonym Full Names: MRE11 homolog A, double strand break repair nuclease
• NCBI Official Symbol: MRE11A
• NCBI Official Synonym Symbols: ATLD; HNGS1; MRE11; MRE11B
• NCBI Protein Information: double-strand break repair protein MRE11A
• UniProt Protein Name: Double-strand break repair protein MRE11A
• Protein Family: Double-strand break repair protein
• UniProt Gene Name: MRE11A
• UniProt Synonym Gene Names: HNGS1; MRE11; MRE11 homolog 1; MRE11 homolog A
• UniProt Entry Name: MRE11_HUMAN

NCBI Description

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

MRE11A: a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Alternative splicing results in two different isoforms.

Protein type: DNA-binding; Deoxyribonuclease; Cell cycle regulation; DNA repair, damage

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: nucleoplasm; chromosome, telomeric region; Mre11 complex; cytosol; chromatin; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; nuclease activity; DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; manganese ion binding; endonuclease activity; double-stranded DNA binding; 3'-5' exonuclease activity; endodeoxyribonuclease activity

Biological Process: sister chromatid cohesion; positive regulation of kinase activity; negative regulation of DNA endoreduplication; telomere maintenance via telomerase; regulation of mitotic recombination; DNA repair; positive regulation of protein amino acid autophosphorylation; DNA catabolic process, endonucleolytic; DNA duplex unwinding; double-strand break repair via nonhomologous end joining; double-strand break repair via homologous recombination; DNA recombination; cell proliferation; intra-S DNA damage checkpoint; base-excision repair; nucleotide-excision repair; mitotic cell cycle G2/M transition DNA damage checkpoint; double-strand break repair; meiotic recombination; synapsis; positive regulation of interferon type I production; innate immune response; telomere maintenance; response to DNA damage stimulus

Disease: Ataxia-telangiectasia-like Disorder 1