Rabbit anti-Human MRAP Polyclonal Antibody | anti-MRAP antibody

MRAP, NT (MRAP, C21orf61, FALP, Melanocortin-2 receptor accessory protein, B27, Fat cell-specific low molecular weight protein, Fat tissue-specific low MW protein) (MaxLight 750)

Cat. Num. AAA6322030

• Gene Names: MRAP; B27; FALP; FGD2; GCCD2; C21orf61
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MRAP; Polyclonal Antibody; NT (MRAP; C21orf61; FALP; Melanocortin-2 receptor accessory protein; B27; Fat cell-specific low molecular weight protein; Fat tissue-specific low MW protein) (MaxLight 750); Fat tissue-specific low MW protein; anti-MRAP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MRAP.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-MRAP antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 7-36 in the N-terminal region of human MRAP, UniProt Accession #Q8TCY5.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis o f293 cell line lysates (35ug/lane) usingMBS642167. MRAP (arrow) was detected usingMBS642167.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded breast carcinoma using MBS642167 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS642167 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis in 293 cells (right histogram) using MBS642167 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MRAP antibody

MRAP encodes a melanocortin receptor-interacting protein. The encoded protein regulates trafficking and function of the melanocortin 2 receptor in the adrenal gland. The encoded protein can also modulate signaling of other melanocortin receptors. Mutations in this gene have been associated with familial glucocorticoid deficiency type 2.

Product Categories/Family for anti-MRAP antibody

Antibodies; Receptors

References

1. Dias, R.P., et al. Eur. J. Endocrinol. 162(2):357-359(2010). 2. Chan, L.F., et al. Proc. Natl. Acad. Sci. U.S.A. 106(15):6146-6151(2009). 3. Webb, T.R., et al. Endocrinology 150(2):720-726(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 45827704
• NCBI GeneID: 56246
• NCBI Accession #: NP_996781.1
• NCBI GenBank Nucleotide #: NM_206898.1
• UniProt Accession #: Q8TCY5; Q5EBR3; Q8TDB7; Q8WXC1; Q8WXC2
• Molecular Weight: 19,136 Da
• NCBI Official Full Name: melanocortin-2 receptor accessory protein isoform beta
• NCBI Official Synonym Full Names: melanocortin 2 receptor accessory protein
• NCBI Official Symbol: MRAP
• NCBI Official Synonym Symbols: B27; FALP; FGD2; GCCD2; C21orf61
• NCBI Protein Information: melanocortin-2 receptor accessory protein; fat tissue-specific low MW protein; fat cell-specific low molecular weight protein
• UniProt Protein Name: Melanocortin-2 receptor accessory protein
• Protein Family: Melanocortin-2 receptor accessory protein
• UniProt Gene Name: MRAP
• UniProt Synonym Gene Names: C21orf61; FALP
• UniProt Entry Name: MRAP_HUMAN

NCBI Description

This gene encodes a melanocortin receptor-interacting protein. The encoded protein regulates trafficking and function of the melanocortin 2 receptor in the adrenal gland. The encoded protein can also modulate signaling of other melanocortin receptors. Mutations in this gene have been associated with familial glucocorticoid deficiency type 2. Alternatively spliced transcript variants have been described. [provided by RefSeq, Dec 2009]

Uniprot Description

MRAP: Required for MC2R expression in certain cell types, suggesting that it is involved in the processing, trafficking or function of MC2R. May be involved in the intracellular trafficking pathways in adipocyte cells. Defects in MRAP are the cause of glucocorticoid deficiency type 2 (GCCD2); also known as familial glucocorticoid deficiency type 2 (FGD2). GCCD2 is an autosomal recessive disorder due to congenital insensitivity or resistance to adrenocorticotropin (ACTH). It is characterized by progressive primary adrenal insufficiency, without mineralocorticoid deficiency. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.1

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; plasma membrane; integral to membrane

Molecular Function: type 5 melanocortin receptor binding; type 3 melanocortin receptor binding; protein binding; type 4 melanocortin receptor binding; adrenocorticotropin hormone receptor binding

Biological Process: positive regulation of cAMP biosynthetic process; brown fat cell differentiation

Disease: Glucocorticoid Deficiency 2