Western Blot (WB)
(Staining (1ug/ml) of HEK293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat MPG Polyclonal Antibody | anti-MPG antibody
Goat anti-MPG Antibody
Gene Names
MPG; AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
MPG; Polyclonal Antibody; Goat anti-MPG Antibody; MDG; N-methylpurine-DNA glycosylase; AAG; APNG; CRA36.1; Mid1; PIG11; PIG16; anpg; 3' end of the Mid1 gene; localized 68 kb upstream the humanzeta globin gene on 16p; CRA36.1 (3-methyl-adenine DNA glycosylase); alkylad; N-methylpurine-DNA glycosylase antibody; AAG antibody; APNG antibody; CRA36.1 antibody; MDG antibody; Mid1 antibody; MPG antibody; PIG11 antibody; PIG16 antibody; anpg antibody; 3\1 end of the Mid1 gene; localized 68 kb upstream the humanzeta globin gene on 16p antibody; CRA36.1 (3-methyl-adenine DNA glycosylase) antibody; alkyladenine DNA glycosylase antibody; proliferation-inducing protein 11 antibody; proliferation-inducing protein 16 antibody; anti-MPG antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SVVDRVAEQDTQA
Sequence Length
298
Applicable Applications for anti-MPG antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 1000.
Western Blot: Approx 37kDa band observed in lysates of HEK293 and K562 (calculated MW of 32.9kDa according to NP_002425.2). Recommended concentration: 1-3ug/ml.
Western Blot: Approx 37kDa band observed in lysates of HEK293 and K562 (calculated MW of 32.9kDa according to NP_002425.2). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-SVVDRVAEQDTQA, from the C Terminus of the protein sequence according to NP_002425.2; NP_001015052.1; NP_001015054.1.
Epitope
C Terminus
Note
This antibody is expected to recognize all three reported isoforms (NP_002425.2, NP_001015052.1 and NP_001015054.1)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
(Staining (1ug/ml) of HEK293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (1ug/ml) of HEK293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,824 Da
NCBI Official Full Name
DNA-3-methyladenine glycosylase isoform a
NCBI Official Synonym Full Names
N-methylpurine DNA glycosylase
NCBI Official Symbol
MPG
NCBI Official Synonym Symbols
AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1
NCBI Protein Information
DNA-3-methyladenine glycosylase
UniProt Protein Name
DNA-3-methyladenine glycosylase
Protein Family
UniProt Gene Name
MPG
UniProt Synonym Gene Names
AAG; ANPG; MID1
UniProt Entry Name
3MG_HUMAN
Uniprot Description
MPG: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. Belongs to the DNA glycosylase MPG family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor co-regulator; EC 3.2.2.21; Hydrolase
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: nucleoplasm
Molecular Function: alkylbase DNA N-glycosylase activity; damaged DNA binding; DNA N-glycosylase activity; DNA-3-methyladenine glycosylase I activity; protein binding
Biological Process: base-excision repair; depurination; DNA dealkylation
Research Articles on MPG
Similar Products
Product Notes
The MPG mpg (Catalog #AAA421319) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MPG Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's MPG can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 1000. Western Blot: Approx 37kDa band observed in lysates of HEK293 and K562 (calculated MW of 32.9kDa according to NP_002425.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the MPG mpg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SVVDRVAEQD TQA. It is sometimes possible for the material contained within the vial of "MPG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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