Western Blot (WB)
((0.3ug/ml) staining of Human Brain (Cerebral Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat MP100/NPEPPS Polyclonal Antibody | anti-NPEPPS antibody
Goat anti-MP100/NPEPPS Antibody
Western Blot: Approx 110kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 98.5kDa according to NP_006301.2). Recommended concentration: 0.2-0.5ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of Human Brain (Cerebral Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Brain (Cerebral Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq, Jul 2008]
Uniprot Description
NPEPPS: Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins. Digests tau from normal brain more efficiently than tau from Alzheimer disease brain. Belongs to the peptidase M1 family.
Protein type: Protease; EC 3.4.11.14
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: cytosol; nucleus; plasma membrane
Molecular Function: aminopeptidase activity; peptide binding; zinc ion binding
Biological Process: peptide catabolic process; protein polyubiquitination; proteolysis
Research Articles on NPEPPS
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Product Notes
The NPEPPS npepps (Catalog #AAA421564) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MP100/NPEPPS Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MP100/NPEPPS can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 110kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 98.5kDa according to NP_006301.2). Recommended concentration: 0.2-0.5ug/ml. Researchers should empirically determine the suitability of the NPEPPS npepps for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KRDAESIHQY LLQRK. It is sometimes possible for the material contained within the vial of "MP100/NPEPPS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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