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Western Blot (WB) (Western Blot: Sample: Recombinant MRP1, Human.)

Rabbit anti-Human Motility Related Protein Polyclonal Antibody | anti-MRP1 antibody

Motility Related Protein (MRP1) Polyclonal Antibody

Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Motility Related Protein; Polyclonal Antibody; Motility Related Protein (MRP1) Polyclonal Antibody; CD9; TSPAN29; 5H9; BA2; BTCC-1; DRAP-27; GIG2; MIC3; P24; Tetraspanin 29; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; anti-MRP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MRP1. It has been selected for its ability to recognize MRP1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MRP1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human
Organism Species
Human
Fragment
MRP1 (Ser112~Ile195)
Quality Control
Content: The quality control contains recombinant MRP1 (Ser112~Ile195) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2051567
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant MRP1, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant MRP1, Human.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:HumanTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:HumanTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
928
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,416 Da
NCBI Official Full Name
CD9 antigen isoform 2
NCBI Official Synonym Full Names
CD9 molecule
NCBI Official Symbol
CD9
NCBI Official Synonym Symbols
MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
NCBI Protein Information
CD9 antigen
UniProt Protein Name
CD9 antigen
Protein Family
UniProt Gene Name
CD9
UniProt Synonym Gene Names
MIC3; TSPAN29; MRP-1; Tspan-29

NCBI Description

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]

Uniprot Description

CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family.

Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: apical plasma membrane; external side of plasma membrane; extracellular space; focal adhesion; integral component of plasma membrane; membrane; plasma membrane; platelet alpha granule membrane

Molecular Function: integrin binding; protein binding

Biological Process: brain development; cell adhesion; cell motility; cell surface receptor signaling pathway; fusion of sperm to egg plasma membrane involved in single fertilization; negative regulation of cell proliferation; oligodendrocyte development; paranodal junction assembly; platelet activation; platelet degranulation; receptor internalization; response to water deprivation

Research Articles on MRP1

Similar Products

Product Notes

The MRP1 cd9 (Catalog #AAA2032615) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Motility Related Protein (MRP1) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Motility Related Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the MRP1 cd9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Motility Related Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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