Rabbit anti-Rat Monocarboxylate Transporter 4 Polyclonal Antibody | anti-SLC16A3 antibody

Monocarboxylate Transporter 4 (MCT4)

Cat. Num. AAA614511

• Gene Names: SLC16A3; MCT3; MCT4; MCT 3; MCT 4; MCT-3; MCT-4; MGC138472; MGC138474
• Reactivity: Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Monocarboxylate Transporter 4; Polyclonal Antibody; Monocarboxylate Transporter 4 (MCT4); Anti -Monocarboxylate Transporter 4 (MCT4); anti-SLC16A3 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Monocarboxylate Transporter 4. No significant sequence homology exists with other MCTs.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA

• Applicable Applications for anti-SLC16A3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Immunohistochemistry and Western Blot.; Dilution: Western Blot: 1-5ug/ml using ECL; ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. ; Immunohistochemistry: 5-10ug Paraformaldehyde fixed.
• Immunogen: Synthetic peptide corresponding to 19aa near the C terminal, cytoplasmic domain of rat MCT, conjugated to KLH. Species Sequence Homology: mouse, human 100%, chicken 78%.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-SLC16A3 antibody

Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner. MCT4/MOT4/SLC16A3 (mouse 470-aa, rat 471-aa, human 465-aa; chromosome 17q25) is most closely related to MCT3, is prominently expressed in skeletal muscle and other cells with a high glycolytic rate such as tumor cells and white blood cells, suggesting an important role in lactic acid efflux. According to new nomenclature, the old MCT3 has now been reclassified as MCT4. This antibody has previously been listed as MCT31-S. Please consult the main MCT page for a detailed nomenclature.

Product Categories/Family for anti-SLC16A3 antibody

Antibodies; Abs to Monocarboxylate Transporter

NCBI and Uniprot Product Information

• NCBI GI #: 109288012
• NCBI GeneID: 9123
• NCBI Accession #: NP_001035887.1
• NCBI GenBank Nucleotide #: NM_001042422.2
• UniProt Accession #: O15427; Q2M1P8; B3KXG8; Q53G91
• Molecular Weight: 49,469 Da
• NCBI Official Full Name: monocarboxylate transporter 4
• NCBI Official Synonym Full Names: solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
• NCBI Official Symbol: SLC16A3
• NCBI Official Synonym Symbols: MCT3; MCT4; MCT 3; MCT 4; MCT-3; MCT-4; MGC138472; MGC138474
• NCBI Protein Information: monocarboxylate transporter 4; monocarboxylate transporter 3; solute carrier family 16 (monocarboxylic acid transporters), member 3
• UniProt Protein Name: Monocarboxylate transporter 4
• Protein Family: Monocarboxylate transporter
• UniProt Gene Name: SLC16A3
• UniProt Synonym Gene Names: MCT4
• UniProt Entry Name: MOT4_HUMAN

NCBI Description

Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family-16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 (SLC16A1; MIM 600682) and MCT2 (SLC16A7; MIM 603654), are characterized by 12 predicted transmembrane domains (Price et al., 1998 [PubMed 9425115]).[supplied by OMIM]

Uniprot Description

SLC16A3: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.

Protein type: Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nuclear membrane; membrane; integral to plasma membrane; plasma membrane; actin cytoskeleton

Molecular Function: monocarboxylic acid transmembrane transporter activity; symporter activity; lactate transmembrane transporter activity; secondary active monocarboxylate transmembrane transporter activity

Biological Process: cellular metabolic process; monocarboxylic acid transport; pyruvate metabolic process; blood coagulation; transmembrane transport; leukocyte migration