anti-Human Monocarboxylate Transporter 1 Polyclonal Antibody | anti-SLC16A1 antibody
Anti - Monocarboxylate Transporter 1 (MCT1)
Gene Names
SLC16A1; MCT; HHF7; MCT1; FLJ36745; MGC44475
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Monocarboxylate Transporter 1; Polyclonal Antibody; Anti - Monocarboxylate Transporter 1 (MCT1); Monocarboxylate Transporter 1 (MCT1); anti-SLC16A1 antibody
Host
Host: Rabbit
Source: Human
Source: Human
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human sequence is 63% conserved in hamster and 57% in mouse. No significant sequence homology was found with other MCTs. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking (use 5-10 ug per 1 ul of antiserum or 1 ug of aff pure IgG) to confirm antibody specificity
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Sequence Length
500
Applicable Applications for anti-SLC16A1 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blot. Other applications not tested.
Dilution: Western Blot: 1-5ug/ml (ECL).
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1-5ug/ml (ECL).
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Immunogen
A 19-aa peptide sequence within the cytoplasmic, C-terminus of human MCT1 (1) was synthesized, coupled to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-SLC16A1 antibody
Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner.
Product Categories/Family for anti-SLC16A1 antibody
References
• 1. Am. J. Physiology Endocriol. Metabol. 1997) 36, E207-E213; Diabetologia (1999) 42, 870-877; J. Biol. Chem. (1999) 274, 284220-284220; Takanaga H et al (1995) BBRC 217, 370-377; Carpenter L et al (1996) Biochim. Biophys. Acta 1279, 157-163; Koehler-Stec EM et al (1998) AM. J. Physiol. 275, E516-E524; Yoon H et al (1999) Genomics 60, 366-370; Halestrap AP and Price NT (1999) Biochem J. 343, 281-299 (review).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,944 Da
NCBI Official Full Name
monocarboxylate transporter 1
NCBI Official Synonym Full Names
solute carrier family 16, member 1 (monocarboxylic acid transporter 1)
NCBI Official Symbol
SLC16A1
NCBI Official Synonym Symbols
MCT; HHF7; MCT1; FLJ36745; MGC44475
NCBI Protein Information
monocarboxylate transporter 1; MCT 1; OTTHUMP00000013960; OTTHUMP00000013961; solute carrier family 16 member 1; solute carrier family 16 (monocarboxylic acid transporters), member 1
UniProt Protein Name
Monocarboxylate transporter 1
Protein Family
UniProt Gene Name
SLC16A1
UniProt Synonym Gene Names
MCT1
UniProt Entry Name
MOT1_HUMAN
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Product Notes
The SLC16A1 slc16a1 (Catalog #AAA613497) is an Antibody produced from Host: Rabbit Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - Monocarboxylate Transporter 1 (MCT1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Monocarboxylate Transporter 1 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blot. Other applications not tested. Dilution: Western Blot: 1-5ug/ml (ECL). ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the SLC16A1 slc16a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Monocarboxylate Transporter 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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