Western Blot (WB)
((2ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat MON1A Polyclonal Antibody | anti-MON1A antibody
Goat anti-MON1A (aa589-601) Antibody
Gene Names
MON1A; SAND1
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
MON1A; Polyclonal Antibody; Goat anti-MON1A (aa589-601) Antibody; Goat Anti-MON1A (aa478-489) Antibody; MON1 homolog A (yeast); FLJ97088; MGC13272; SAND1; MON1 homolog A; FLJ97088 antibody; MGC13272 antibody; MON1 homolog A antibody; MON1 homolog A (yeast) antibody; SAND1 antibody; MON1A antibody; MON1A (aa589-601); anti-MON1A antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RPLKTIYYTGPNE
Sequence Length
652
Applicable Applications for anti-MON1A antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 50kDa band observed in lysates of cell line Jurkat (calculated MW of 55.5kDa according to NP_001135973.1). Recommended concentration: 1-3ug/ml.
Western Blot: Approx 50kDa band observed in lysates of cell line Jurkat (calculated MW of 55.5kDa according to NP_001135973.1). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-RPLKTIYYTGPNE, from the internal region of the protein sequence according to NP_115731.2; NP_001135973.1.
Epitope
Internal region
Note
This antibody is expected to recognize isoforms a and b (NP_115731.2; NP_001135973.1). Amino acid numbering in name refers to sequence NP_115731.2.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((2ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,456 Da
NCBI Official Full Name
vacuolar fusion protein MON1 homolog A isoform a
NCBI Official Synonym Full Names
MON1 homolog A, secretory trafficking associated
NCBI Official Symbol
MON1A
NCBI Official Synonym Symbols
SAND1
NCBI Protein Information
vacuolar fusion protein MON1 homolog A
UniProt Protein Name
Vacuolar fusion protein MON1 homolog A
Protein Family
UniProt Gene Name
MON1A
UniProt Synonym Gene Names
SAND1
UniProt Entry Name
MON1A_HUMAN
Uniprot Description
MON1A: Plays an important in membrane trafficking through the secretory apparatus. Not involved in endocytic trafficking to lysosomes. Belongs to the MON1/SAND family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: endomembrane system
Biological Process: protein secretion; vesicle-mediated transport
Research Articles on MON1A
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Product Notes
The MON1A mon1a (Catalog #AAA422296) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MON1A (aa589-601) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MON1A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 50kDa band observed in lysates of cell line Jurkat (calculated MW of 55.5kDa according to NP_001135973.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the MON1A mon1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RPLKTIYYTG PNE. It is sometimes possible for the material contained within the vial of "MON1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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