Rabbit anti-Human MOCS2 Polyclonal Antibody | anti-MOCS2 antibody

MOCS2 Antibody

Cat. Num. AAA7046695

• Gene Names: MOCS2; MPTS; MCBPE; MOCO1; MOCODB
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Antigen Affinity Purified
• Synonyms: MOCS2; Polyclonal Antibody; MOCS2 Antibody; Molybdopterin synthase sulfur carrier subunit; MOCO1-A; Molybdenum cofactor synthesis protein 2 small subunit; MOCO1; anti-MOCS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 88

• Applicable Applications for anti-MOCS2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Species: Human
• Immunogen: Recombinant human Molybdopterin synthase sulfur carrier subunit protein (1-188AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: MOCS2 antibody at 0.47 ug/mlLane 1: A549 whole cell lysateLane 2: HepG-2 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 21 kDaObserved band size: 21,49 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7046695 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7046695 at dilution of 1:100)

Immunoprecipitation (IP)

(Immunoprecipitating MOCS2 in Jurkat whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS7046695 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)Lane 2: MBS7046695(4ug)+ Jurkat whole cell lysate(500ug)Lane 3: Jurkat whole cell lysate (20ug))

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells cells using MBS7046695 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-MOCS2 antibody

Acts as a sulfur carrier required for molybdopterin biosynthesis. Component of the molybdopterin synthase complex that catalyzes the conversion of precursor Z into molybdopterin by mediating the incorporation of 2 sulfur atoms into precursor Z to generate a dithiolene group. In the complex, serves as sulfur donor by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. After interaction with MOCS2B, the sulfur is then transferred to precursor Z to form molybdopterin.

NCBI and Uniprot Product Information

• NCBI GI #: 28631173
• NCBI GeneID: 4338
• NCBI Accession #: NP_789776.1
• NCBI GenBank Nucleotide #: NM_176806.3
• UniProt Accession #: O96033; O96007
• Molecular Weight: 9,755 Da
• NCBI Official Full Name: molybdopterin synthase sulfur carrier subunit small subunit MOCS2A
• NCBI Official Synonym Full Names: molybdenum cofactor synthesis 2
• NCBI Official Symbol: MOCS2
• NCBI Official Synonym Symbols: MPTS; MCBPE; MOCO1; MOCODB
• NCBI Protein Information: molybdopterin synthase catalytic subunit; molybdopterin synthase sulfur carrier subunit
• UniProt Protein Name: Molybdopterin synthase sulfur carrier subunit
• UniProt Gene Name: MOCS2
• UniProt Synonym Gene Names: MOCS2A
• UniProt Entry Name: MOC2A_HUMAN

NCBI Description

Eukaryotic molybdoenzymes use a unique molybdenum cofactor (MoCo) consisting of a pterin, termed molybdopterin, and the catalytically active metal molybdenum. MoCo is synthesized from precursor Z by the heterodimeric enzyme molybdopterin synthase. The large and small subunits of molybdopterin synthase are both encoded from this gene by overlapping open reading frames. The proteins were initially thought to be encoded from a bicistronic transcript. They are now thought to be encoded from monocistronic transcripts. Alternatively spliced transcripts have been found for this locus that encode the large and small subunits. [provided by RefSeq, Jul 2008]

Uniprot Description

MOCS2 iso2: Catalytic subunit of the molybdopterin synthase complex, a complex that catalyzes the conversion of precursor Z into molybdopterin. Acts by mediating the incorporation of 2 sulfur atoms from thiocarboxylated MOCS2A into precursor Z to generate a dithiolene group. Defects in MOCS2 are the cause of molybdenum cofactor deficiency type B (MOCOD type B). MOCOD type B is an autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death. Belongs to the MoaE family. MOCS2B subfamily.

Protein type: EC 2.8.1.12; Enzyme, misc.

Chromosomal Location of Human Ortholog: 5q11

Cellular Component: cytoplasm; cytosol; molybdopterin synthase complex; nucleus

Molecular Function: Mo-molybdopterin synthase activity

Biological Process: Mo-molybdopterin cofactor biosynthetic process; molybdopterin cofactor biosynthetic process

Disease: Molybdenum Cofactor Deficiency, Complementation Group A