Western Blot (WB)
(Western blot analysis of lysates from MCF-7,HepG2 cell line (from left to right),using Mnk1 Antibody (Ab-385)(B8376). B8376 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Rabbit Mnk1 Polyclonal Antibody | anti-MKNK1 antibody
Mnk1 Antibody (Ab-385)
Western Blot (WB)
(Western blot analysis of lysates from MCF-7,HepG2 cell line (from left to right),using Mnk1 Antibody (Ab-385)(B8376). B8376 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from MCF-7,HepG2 cell line (from left to right),using Mnk1 Antibody (Ab-385)(B8376). B8376 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of extracts from, K562 cells (Lane 1) and Jurket cells (Lane 2), using Mnk1 (Ab-385) Antibody. The lane on the left is treated with systhesized peptide.)
Western Blot (WB)
(Western blot analysis of extracts from, K562 cells (Lane 1) and Jurket cells (Lane 2), using Mnk1 (Ab-385) Antibody. The lane on the left is treated with systhesized peptide.)
Knauf U.,et al.Mol. Cell. Biol. 21:5500-5511(2001).
O'Loghlen A.,et al.Exp. Cell Res. 299:343-355(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
Mnk1: a CAM kinase of the MAPKKAPK family that can regulate translation. Following activation by MAP kinases, Mnk1 phosphorylates eIF4E at Ser209, increasing its affinity for the 7-methylguanosine-containing mRNA cap. Two MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for its kinase activity. Three alternatively spliced human isoforms have been described.
Protein type: Protein kinase, CAMK; Translation; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; MAPKAPK family; MNK subfamily
Chromosomal Location of Human Ortholog: 1p33
Cellular Component: nucleus; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding
Biological Process: peptidyl-serine phosphorylation; fibroblast growth factor receptor signaling pathway; protein amino acid phosphorylation; response to salt stress; regulation of translational initiation
Research Articles on MKNK1
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Product Notes
The MKNK1 mknk1 (Catalog #AAA9214485) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 365-395. The Mnk1 Antibody (Ab-385) reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Mnk1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~ 1:250-1:1000. Researchers should empirically determine the suitability of the MKNK1 mknk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mnk1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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