Rabbit MNAT1 Polyclonal Antibody | anti-MNAT1 antibody

MNAT1 (Monomorphic Arylamine N-acetyltransferase, MAT1, TFB3, CAP35, RNF66) (FITC)

Cat. Num. AAA6427038

• Gene Names: MNAT1; MAT1; TFB3; CAP35; RNF66
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: MNAT1; Polyclonal Antibody; MNAT1 (Monomorphic Arylamine N-acetyltransferase; MAT1; TFB3; CAP35; RNF66) (FITC); anti-MNAT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes MNAT1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-MNAT1 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human MNAT1.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MNAT1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using MNAT1 antibody at dilution of 1:200 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cell using MNAT1 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-MNAT1 antibody

Antibodies; Enzymes; Acetyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 295789085
• NCBI GeneID: 4331
• NCBI Accession #: NP_001171434.1
• NCBI GenBank Nucleotide #: NM_001177963.1
• UniProt Accession #: P51948; Q15817; Q6ICQ7
• Molecular Weight: 35,823 Da
• NCBI Official Full Name: CDK-activating kinase assembly factor MAT1 isoform 2
• NCBI Official Synonym Full Names: menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis)
• NCBI Official Symbol: MNAT1
• NCBI Official Synonym Symbols: MAT1; TFB3; CAP35; RNF66
• NCBI Protein Information: CDK-activating kinase assembly factor MAT1; RING finger protein 66; RING finger protein MAT1; CDK7/cyclin-H assembly factor; cyclin G1 interacting protein; cyclin-G1-interacting protein; menage a trois 1 (CAK assembly factor)
• UniProt Protein Name: CDK-activating kinase assembly factor MAT1
• Protein Family: CDK-activating kinase assembly factor
• UniProt Gene Name: MNAT1
• UniProt Synonym Gene Names: CAP35; MAT1; RNF66
• UniProt Entry Name: MAT1_HUMAN

NCBI Description

The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

MNAT1: Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.

Protein type: Nuclear receptor co-regulator; Cell cycle regulation; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 14q23

Cellular Component: nucleoplasm; holo TFIIH complex; cytoplasm

Molecular Function: RNA polymerase subunit kinase activity; DNA-dependent ATPase activity; protein binding; zinc ion binding; protein N-terminus binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; positive regulation of smooth muscle cell proliferation; protein amino acid phosphorylation; regulation of gene expression, epigenetic; mRNA capping; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; protein complex assembly; nucleotide-excision repair, DNA damage removal; G2/M transition of mitotic cell cycle; adult heart development; transcription initiation from RNA polymerase II promoter; ventricular system development; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; termination of RNA polymerase I transcription; DNA repair; regulation of transcription from RNA polymerase II promoter; cell proliferation; nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; response to calcium ion; transcription initiation from RNA polymerase I promoter; G1/S transition of mitotic cell cycle; negative regulation of apoptosis