Rabbit anti-Human, Mouse MMP8 Polyclonal Antibody | anti-MMP8 antibody

MMP8, ID (Matrix Metalloproteinase 8) (MaxLight 650)

Cat. Num. AAA6487004

• Gene Names: MMP8; HNC; CLG1; MMP-8; PMNL-CL
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: MMP8; Polyclonal Antibody; ID (Matrix Metalloproteinase 8) (MaxLight 650); anti-MMP8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MMP8. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-MMP8 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 286-315 in the central region of human MMP8, UniProt Accession #NP_002415; P22894.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis in mouse liver tissue lysate (35ug/lane) usingMBS625586. MMP8 (arrow) was detected using the purified Pab)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS625586, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS625586 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-MMP8 antibody

Antibodies; Matrix Metalloproteinases

References

1. Van den Steen, P.E., et al., Biochem. Biophys. Res. Commun. 310(3):889-896 (2003). 2. Giambernardi, T.A., et al., Matrix Biol. 20(8):577-587 (2001). 3. Nagase, H., et al., J. Biol. Chem. 274(31):21491-21494 (1999). 4. Massova, I., et al., FASEB J. 12(12):1075-1095 (1998). 5. Pendas, A.M., et al., Genomics 37(2):266-268 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4505221
• NCBI GeneID: 4317
• NCBI Accession #: NP_002415
• NCBI GenBank Nucleotide #: NM_002424.2
• UniProt Accession #: P22894; Q45F99
• Molecular Weight: 44.3 kDa (390aa)
• NCBI Official Full Name: neutrophil collagenase isoform 1 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 8
• NCBI Official Symbol: MMP8
• NCBI Official Synonym Symbols: HNC; CLG1; MMP-8; PMNL-CL
• NCBI Protein Information: neutrophil collagenase
• UniProt Protein Name: Neutrophil collagenase
• Protein Family: Neutrophil collagenase
• UniProt Gene Name: MMP8
• UniProt Synonym Gene Names: CLG1; MMP-8; PMNL-CL
• UniProt Entry Name: MMP8_HUMAN

NCBI Description

This gene encodes a member of the matrix metalloproteinase (MMP) family of proteins. These proteins are involved in the breakdown of extracellular matrix in embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Proteolysis at different sites on this protein results in multiple active forms of the enzyme with distinct N-termini. This protein functions in the degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.

Protein type: Secreted; EC 3.4.24.34; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; serine-type endopeptidase activity; calcium ion binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis