Rabbit anti-Human MMP3 Polyclonal Antibody | anti-MMP3 antibody

MMP3, ID (Stromelysin-1, SL-1, Matrix Metalloproteinase-3, MMP-3, Transin-1, STMY1) (AP)

Cat. Num. AAA6486980

• Gene Names: MMP3; SL-1; STMY; STR1; CHDS6; MMP-3; STMY1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MMP3; Polyclonal Antibody; ID (Stromelysin-1; SL-1; Matrix Metalloproteinase-3; MMP-3; Transin-1; STMY1) (AP); anti-MMP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MMP3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-MMP3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human MMP3 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MMP3 (arrow) using rabbit polyclonal MMP3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP3 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using MMP3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-MMP3 antibody

Antibodies; Matrix Metalloproteinases

References

1. Sage, E.H., et al., J. Biol. Chem. 278(39):37849-37857 (2003). 2. Matsuyama, A., et al., Circulation 108(12):1469-1473 (2003). 3. Mercapide, J., et al., Int. J. Cancer 106(5):676-682 (2003). 4. Bodemer, C., et al., J. Invest. Dermatol. 121(2):273-279 (2003). 5. Kang, M.K., et al., Exp. Cell Res. 287(2):272-281 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4505217
• NCBI GeneID: 4314
• NCBI Accession #: NP_002413
• NCBI GenBank Nucleotide #: NM_002422.4
• UniProt Accession #: P08254; Q3B7S0; Q6GRF8; B2R8B8
• Molecular Weight: 45.2 kDa (401aa) confirmed by MALDI-TOF
• NCBI Official Full Name: stromelysin-1 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 3
• NCBI Official Symbol: MMP3
• NCBI Official Synonym Symbols: SL-1; STMY; STR1; CHDS6; MMP-3; STMY1
• NCBI Protein Information: stromelysin-1
• UniProt Protein Name: Stromelysin-1
• Protein Family: Stromelysin
• UniProt Gene Name: MMP3
• UniProt Synonym Gene Names: STMY1; SL-1; MMP-3
• UniProt Entry Name: MMP3_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Catalytic activity: Preferential cleavage where P1', P2' and P3' are hydrophobic residues.

Cofactor: Binds 4 calcium ions per subunit.Binds 2 zinc ions per subunit.

Subcellular location: Secreted › extracellular space › extracellular matrix

Probable.

Domain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Ref.13

Involvement in disease: Coronary heart disease 6 (CHDS6) [MIM:614466]: A multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Decreased capacity of the coronary vessels is often associated with thickening and loss of elasticity of the coronary arteries.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. A polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infarction, due to lower MMP3 proteolytic activity and higher extracellular matrix deposition in atherosclerotic lesions. Ref.11 Ref.12

Sequence similarities: Belongs to the peptidase M10A family.Contains 4 hemopexin-like domains.