Rabbit MMP20 Polyclonal Antibody | anti-MMP20 antibody

MMP20 Polyclonal Antibody

Cat. Num. AAA3006961

• Gene Names: MMP20; AI2A2; MMP-20
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MMP20; Polyclonal Antibody; MMP20 Polyclonal Antibody; Matrix metalloproteinase-20; MMP-20; Enamel metalloproteinase; Enamelysin; anti-MMP20 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MMP20 polyclonal antibody detects endogenous levels of MMP20 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 483

• Applicable Applications for anti-MMP20 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human MMP20
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MMP20 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysate Lane3:PC12 whole cell lysate)

Related Product Information for anti-MMP20 antibody

Matrix metalloproteinases (MMPs) are highly homologous Zn2+ endopeptidases involved in extracellular matrix (ECM) breakdown. MMP-20 (enamelysin) is involved in the degradation of various components of the ECM during development, hemostasis and pathological conditions. The domain organization of MMP-20 is similar to other MMPs, including a signal peptide, a prodomain with the conserved motif PRCGVPD involved in maintaining enzyme latency, a catalytic domain with a Zn-binding site, and a COOH-terminal fragment similar to the sequence of hemopexin. MMP-20 is expressed during the early through middle stages of enamel development at which time it likely hydrolyzes Amelogenin, a major protein component of the enamel matrix. The expression pattern of MMP-20 in the enamel organ indicates that it may be involved in the turnover of ECM proteins during tooth development and enamel formation. Human MMP-20 maps to chromosome 11q22.3, clustered to at least seven other members of the MMP gene family.

NCBI and Uniprot Product Information

• NCBI GI #: 45359865
• NCBI GeneID: 9313
• NCBI Accession #: NP_004762.2
• NCBI GenBank Nucleotide #: NP_004762.2
• UniProt Accession #: O60882
• Molecular Weight: ~ 54kDa
• NCBI Official Full Name: matrix metalloproteinase-20 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 20
• NCBI Official Symbol: MMP20
• NCBI Official Synonym Symbols: AI2A2; MMP-20
• NCBI Protein Information: matrix metalloproteinase-20
• UniProt Protein Name: Matrix metalloproteinase-20
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP20
• UniProt Synonym Gene Names: MMP-20

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and thus thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes located on chromosome 11q22.3. [provided by RefSeq, Aug 2011]

Uniprot Description

Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. Cleaves aggrecan at the '360-Asn-|-Phe-361' site.