Western Blot (WB)
(Western blot analysis of anti-MMP20 Antibody (Center) in HepG2 cell line lysates (35ug/lane). MMP20 (arrow) was detected using the purified Pab.)
Rabbit anti-Human MMP20 Polyclonal Antibody | anti-MMP20 antibody
MMP20 Antibody (Center)
Western Blot (WB)
(Western blot analysis of anti-MMP20 Antibody (Center) in HepG2 cell line lysates (35ug/lane). MMP20 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-MMP20 Antibody (Center) in HepG2 cell line lysates (35ug/lane). MMP20 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Stracke, J.O., et al., FEBS Lett. 478 (1-2), 52-56 (2000).
NCBI and Uniprot Product Information
NCBI Description
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and thus thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes located on chromosome 11q22.3. [provided by RefSeq, Aug 2011]
Uniprot Description
MMP20: Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2). AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel. Belongs to the peptidase M10A family.
Protein type: Protease; EC 3.4.24.-; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 11q22.3
Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region
Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; calcium ion binding
Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; protein catabolic process; proteolysis
Disease: Amelogenesis Imperfecta, Hypomaturation Type, Iia2
Research Articles on MMP20
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Product Notes
The MMP20 mmp20 (Catalog #AAA9209246) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 233-262. The MMP20 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MMP20 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the MMP20 mmp20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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