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Western Blot (WB) (Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5% SDS PAGE gel and MMP2 antibody at a dilution of 1:1000)

Rabbit MMP2 Polyclonal Antibody | anti-MMP2 antibody

MMP2 Antibody

Gene Names
MMP2; CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
MMP2; Polyclonal Antibody; MMP2 Antibody; Affinity Purified Rabbit Polyclonal MMP2 Antibody; Polyclonal MMP2 antibody; Anti-MMP2 antibody; 72 kDa type IV collagenase antibody; MONA antibody; MMP-II antibody; gelatinase A antibody; MMP-2; 72 kDa gelatinase antibody; matrix metalloproteinase-II antibody; TBE-1 antibody; CLG4A antibody; MMP2 antibody; CLG4 antibody; MMP 2; matrix metalloproteinase-2 antibody; MMP 2 antibody; collagenase type IV-A antibody; MMP-2 antibody; anti-MMP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.55 mg/ml (varies by lot)
Sequence Length
61
Applicable Applications for anti-MMP2 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
IF: 1:100-1:500
ICC: 1:100-1:500
WB: 1:500-1:3000
Immunogen
MMP2 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 193 and 575 of MMP2
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5% SDS PAGE gel and MMP2 antibody at a dilution of 1:1000)

Western Blot (WB) (Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5% SDS PAGE gel and MMP2 antibody at a dilution of 1:1000)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded U251 xenograft using MMP2 antibody at a dilution of 1:500)

Immunohistochemistry (IHC) (Immunohistochemical staining of paraffin-embedded U251 xenograft using MMP2 antibody at a dilution of 1:500)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 7.5% SDS PAGE gel and MMP2 antibody at a dilution of 1:1000)

Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 7.5% SDS PAGE gel and MMP2 antibody at a dilution of 1:1000)

Immunofluorescence (IF)

(Immunofluorescence analysis of methanol-fixed HeLa, using MMP2 antibody at 1:200 dilution.)

Immunofluorescence (IF) (Immunofluorescence analysis of methanol-fixed HeLa, using MMP2 antibody at 1:200 dilution.)
Related Product Information for anti-MMP2 antibody
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response.
Product Categories/Family for anti-MMP2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
74 kDa
NCBI Official Full Name
MMP2, partial
NCBI Official Synonym Full Names
matrix metallopeptidase 2
NCBI Official Symbol
MMP2
NCBI Official Synonym Symbols
CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
NCBI Protein Information
72 kDa type IV collagenase
Protein Family

NCBI Description

This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Research Articles on MMP2

Similar Products

Product Notes

The MMP2 (Catalog #AAA5311955) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MMP2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB). IF: 1:100-1:500 ICC: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the MMP2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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