Rabbit anti-Human MMP17 Polyclonal Antibody | anti-MMP17 antibody

MMP17, ID (Matrix Metalloproteinase-17, MMP-17, Membrane-type Matrix Metalloproteinase 4, MT-MMP 4, MTMMP4, Membrane-type-4 Matrix Metalloproteinase, MT4-MMP, MT4MMP, MT4MMP) (MaxLight 490)

Cat. Num. AAA6487145

• Gene Names: MMP17; MT4-MMP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: MMP17; Polyclonal Antibody; ID (Matrix Metalloproteinase-17; MMP-17; Membrane-type Matrix Metalloproteinase 4; MT-MMP 4; MTMMP4; Membrane-type-4 Matrix Metalloproteinase; MT4-MMP; MT4MMP; MT4MMP) (MaxLight 490); anti-MMP17 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MMP17.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight490

• Applicable Applications for anti-MMP17 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 391-420 in the central region of human MMP17, UniProt Accession #NP_057239; Q9ULZ9; Q9R0S3. Species sequence homology: mouse
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS629979. MMP17 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS629979, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-MMP17 antibody

Antibodies; Matrix Metalloproteinases

References

1. Jung, M., et al., Prostate 55(2):89-98 (2003). 2. Itoh, Y., et al., J. Biol. Chem. 274 (48):34260-34266 (1999). 3. Wang, Y., et al., J. Biol. Chem. 274(46):33043-33049 (1999). 4. Kajita, M., et al., FEBS Lett. 457(3):353-356 (1999). 5. Nagase, H., et al., J. Biol. Chem. 274(31):21491-21494 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 112382270
• NCBI GeneID: 4326
• NCBI Accession #: NP_057239
• NCBI GenBank Nucleotide #: NM_016155
• UniProt Accession #: Q9ULZ9; Q9R0S3
• Molecular Weight: 57,938 Da
• NCBI Official Full Name: matrix metalloproteinase-17 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 17 (membrane-inserted)
• NCBI Official Symbol: MMP17
• NCBI Official Synonym Symbols: MT4-MMP
• NCBI Protein Information: matrix metalloproteinase-17; MMP-17; MT-MMP 4; MT4MMP; MTMMP4; matrix metalloproteinase 17 (membrane-inserted); membrane-type matrix metalloproteinase 4; membrane-type-4 matrix metalloproteinase
• UniProt Protein Name: Matrix metalloproteinase-17
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP17
• UniProt Synonym Gene Names: MT4MMP; MMP-17; MT-MMP 4; MTMMP4; MT4-MMP; MT4MMP
• UniProt Entry Name: MMP17_HUMAN

Uniprot Description

MMP17: Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin. Belongs to the peptidase M10A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Activator; Protease; Membrane protein, integral; EC 3.4.24.-; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: proteinaceous extracellular matrix; integral to plasma membrane

Molecular Function: zinc ion binding; metalloendopeptidase activity; enzyme activator activity; calcium ion binding

Biological Process: positive regulation of catalytic activity; proteolysis