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Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Mouse stomach tissue, Mouse heart tissue, Mouse liver tissue, HT29 cells, Primary antibody: 820991(MMP15 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Rabbit anti-Human, Mouse MMP15 Polyclonal Antibody | anti-MMP15 antibody

MMP15 Rabbit pAb

Gene Names
MMP15; MMP-15; MT2MMP; MTMMP2; SMCP-2; MT2-MMP
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity purified
Synonyms
MMP15; Polyclonal Antibody; MMP15 Rabbit pAb; matrix metallopeptidase 15 (membrane-inserted); MTMMP2; SMCP-2; MT2-MMP; anti-MMP15 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-MMP15 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Fusion protein of human MMP15
Conjugation
Unconjugated
Modification
Unmodified
Cell Position
Membrane
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Mouse stomach tissue, Mouse heart tissue, Mouse liver tissue, HT29 cells, Primary antibody: 820991(MMP15 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Mouse stomach tissue, Mouse heart tissue, Mouse liver tissue, HT29 cells, Primary antibody: 820991(MMP15 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Product Categories/Family for anti-MMP15 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
75,807 Da
NCBI Official Full Name
Homo sapiens matrix metallopeptidase 15 (membrane-inserted), mRNA
NCBI Official Synonym Full Names
matrix metallopeptidase 15
NCBI Official Symbol
MMP15
NCBI Official Synonym Symbols
MMP-15; MT2MMP; MTMMP2; SMCP-2; MT2-MMP
NCBI Protein Information
matrix metalloproteinase-15
UniProt Protein Name
Matrix metalloproteinase-15
Protein Family
UniProt Gene Name
MMP15
UniProt Synonym Gene Names
MMP-15; MT-MMP 2; MTMMP2; MT2-MMP; MT2MMP
UniProt Entry Name
MMP15_HUMAN

NCBI Description

This gene encodes a member of the peptidase M10 family and membrane-type subfamily of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Members of this subfamily contain a transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. The encoded preproprotein is proteolytically processed to generate the mature protease. This protein may play a role in cancer progression. [provided by RefSeq, Jan 2016]

Uniprot Description

MMP15: Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.-; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular matrix; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; enzyme activator activity; calcium ion binding

Biological Process: positive regulation of catalytic activity; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; protein modification process; proteolysis; response to estradiol stimulus

Research Articles on MMP15

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Product Notes

The MMP15 mmp15 (Catalog #AAA4757824) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MMP15 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MMP15 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the MMP15 mmp15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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