Testing Data
Rabbit anti-Human MMP-13 Polyclonal Antibody | anti-MMP-13 antibody
Anti-MMP-13 (Collagenase-3) Aminoterminal End active MMP-13 Antibody
Gene Names
MMP13; MMP-13
Reactivity
Human
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
MMP-13; Polyclonal Antibody; Anti-MMP-13 (Collagenase-3) Aminoterminal End active MMP-13 Antibody; anti-MMP-13 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody recognizes the latent proenzyme, at 60 Kd, as well as the active form at 48 Kd, and intermediate activation forms. It does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc).
Purity/Purification
Immunogen Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
140
Applicable Applications for anti-MMP-13 antibody
Western Blot (WB)
Application Notes
Anti-Collagenase-3 works well for Western Blotting applications, and when used against the reduced protein identifies a band at 60 Kd. Collagenase-3 was first identified in tumor cells, but has since been found in synoviocytes and normal fibroblasts stimulated by IL-6 or a combination of TNF-a and IL-1. In rodents, MMP-13 is thought to fill the role of MMP-1, which they appear to lack. Human tissues may express both MMP-1 and MMP-13. The low endogenous protein levels may require concentration of samples by ultrafiltration or ammonium sulfate precipitation prior to Western blotting. A recommended starting concentration for Western blots is 1:3,000 when using colorimetric substrates such as BCIP/NBT, and 1:6,000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples.
Immunogen
Synthetic peptide corresponding to the aminoterminal end of active human MMP13.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-MMP-13 antibody
Description: The antibody is a Rabbit antibody to Collagenase-3 (MMP-13), made to a synthetic peptide based on the aminoterminal end of active human MMP-13. The antibody binds to Collagenase-3, but does not cross react with the other MMP family members (MMP-1, MMP-2, MMP-3, MMP-9). The antibody recognizes the latent proenzyme, at 60 Kd, as well as the active form at 48 Kd, and intermediate activation forms. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative and 50% glycerol as cryoprotectant.
Relevance: Collagenase 3 (MMP13) is a secreted 452 residue protein which is released from cells as aninactive zymogen and activated extracellularly by removal of its propeptide. MMP13 was first identified in tumor cells but has since been found in synoviocytes and normal fibroblasts stimulated by IL6 or a combination of TNF alpha and IL1. Under normal conditions, MMP13 is expressed during embryogenesis (fetal bone development) and is present only at very low levels in adult tissue. However, this enzyme is reported to be involved in the development and metastasis of breast and lung carcinomas, chondrosarcomas and osteosarcomas, head and neck carcinomas and some forms of skin cancer. Additionally, this enzyme plays an important role in degenerative bone diseases including osteoarthritis and rheumatoid arthritis. In rodents, MMP13 is thought to fill the role of MMP1, which they appear to lack.
Relevance: Collagenase 3 (MMP13) is a secreted 452 residue protein which is released from cells as aninactive zymogen and activated extracellularly by removal of its propeptide. MMP13 was first identified in tumor cells but has since been found in synoviocytes and normal fibroblasts stimulated by IL6 or a combination of TNF alpha and IL1. Under normal conditions, MMP13 is expressed during embryogenesis (fetal bone development) and is present only at very low levels in adult tissue. However, this enzyme is reported to be involved in the development and metastasis of breast and lung carcinomas, chondrosarcomas and osteosarcomas, head and neck carcinomas and some forms of skin cancer. Additionally, this enzyme plays an important role in degenerative bone diseases including osteoarthritis and rheumatoid arthritis. In rodents, MMP13 is thought to fill the role of MMP1, which they appear to lack.
NCBI and Uniprot Product Information
Research Articles on MMP-13
Similar Products
Product Notes
The MMP-13 (Catalog #AAA4157314) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MMP-13 (Collagenase-3) Aminoterminal End active MMP-13 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MMP-13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Anti-Collagenase-3 works well for Western Blotting applications, and when used against the reduced protein identifies a band at 60 Kd. Collagenase-3 was first identified in tumor cells, but has since been found in synoviocytes and normal fibroblasts stimulated by IL-6 or a combination of TNF-an and IL-1. In rodents, MMP-13 is thought to fill the role of MMP-1, which they appear to lack. Human tissues may express both MMP-1 and MMP-13. The low endogenous protein levels may require concentration of samples by ultrafiltration or ammonium sulfate precipitation prior to Western blotting. A recommended starting concentration for Western blots is 1:3,000 when using colorimetric substrates such as BCIP/NBT, and 1:6,000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. Researchers should empirically determine the suitability of the MMP-13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP-13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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