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Western Blot (WB) (All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilutionLane 1: mouse liver lysateLane 2: mouse lung lysateLane 3: mouse testis lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Mouse Mlkl Polyclonal Antibody | anti-Mlkl antibody

Mlkl Antibody (C-term)

Gene Names
Mlkl; 9130019I15Rik
Reactivity
Mouse
Applications
Western Blot, ELISA
Synonyms
Mlkl; Polyclonal Antibody; Mlkl Antibody (C-term); Mixed lineage kinase domain-like protein; Mlkl {ECO:0000312|EMBL:AAH237551; ECO:0000312|MGI:MGI:1921818}; anti-Mlkl antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This Mouse Mlkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of mouse Mlkl.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
464
Applicable Applications for anti-Mlkl antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000
Antigen Source
Human
Antigen Region
444-472
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilutionLane 1: mouse liver lysateLane 2: mouse lung lysateLane 3: mouse testis lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilutionLane 1: mouse liver lysateLane 2: mouse lung lysateLane 3: mouse testis lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-Mlkl Antibody (C-term)at 1:2000 dilution + NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 54 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-Mlkl Antibody (C-term)at 1:2000 dilution + NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 54 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-Mlkl Antibody (C-term) at 1:1000 dilutionLane 1: mouse lung lysatesLane 2: mouse spleen lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 54 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-Mlkl Antibody (C-term) at 1:1000 dilutionLane 1: mouse lung lysatesLane 2: mouse spleen lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 54 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Mlkl antibody
Description: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Background: The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.
Product Categories/Family for anti-Mlkl antibody
References
Bisson, N., et al. Cell Cycle 7(7):909-916(2008)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54317
NCBI Official Full Name
mixed lineage kinase domain-like protein isoform 2
NCBI Official Synonym Full Names
mixed lineage kinase domain-like
NCBI Official Symbol
Mlkl
NCBI Official Synonym Symbols
9130019I15Rik
NCBI Protein Information
mixed lineage kinase domain-like protein
UniProt Protein Name
Mixed lineage kinase domain-like protein
UniProt Gene Name
Mlkl
UniProt Entry Name
MLKL_MOUSE

NCBI Description

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]

Research Articles on Mlkl

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Product Notes

The Mlkl mlkl (Catalog #AAA9232164) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mlkl Antibody (C-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Mlkl can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000. Researchers should empirically determine the suitability of the Mlkl mlkl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mlkl, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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