Western Blot (WB)
(Western blot analysis of MLH1 expression in KNRK (A); HeLa (B); A431 (C); SW480 (D) whole cell lysates.)
Rabbit MLH1 Polyclonal Antibody | anti-MLH1 antibody
MLH1 Antibody
Gene Names
MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Immunogen Affinity Chromatography
Synonyms
MLH1; Polyclonal Antibody; MLH1 Antibody; Purified Polyclonal MLH1 Antibody; Polyclonal MLH1 antibody; Anti-MLH1 antibody; COCA2 antibody; DNA mismatch repair protein Mlh1 antibody; MutL protein homolog 1 antibody; anti-MLH1 antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of MLH1 protein
Purity/Purification
Immunogen Affinity Chromatography
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Sequence Length
34
Applicable Applications for anti-MLH1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:1000
IF: 1:100-1:500
ICC: 1:100-1:500
IF: 1:100-1:500
ICC: 1:100-1:500
Immunogen
MLH1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MLH1 as the immunogen
Preparation and Storage
Store at 4 degree C for short term storage. For long term, aliquot and store at -20 degree C. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of MLH1 expression in KNRK (A); HeLa (B); A431 (C); SW480 (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of MLH1 expression in KNRK (A); HeLa (B); A431 (C); SW480 (D) whole cell lysates.)
Related Product Information for anti-MLH1 antibody
MLH1 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene, mutL, which mediates protein-protein interactions during mismatch recognition, strand discrimination, and strand removal.
Product Categories/Family for anti-MLH1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
MLH1, partial
NCBI Official Synonym Full Names
mutL homolog 1
NCBI Official Symbol
MLH1
NCBI Official Synonym Symbols
FCC2; COCA2; HNPCC; hMLH1; HNPCC2
NCBI Protein Information
DNA mismatch repair protein Mlh1
Protein Family
NCBI Description
The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]
Research Articles on MLH1
Similar Products
Product Notes
The MLH1 (Catalog #AAA5314681) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MLH1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:1000 IF: 1:100-1:500 ICC: 1:100-1:500. Researchers should empirically determine the suitability of the MLH1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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